| Detailed Information for Phage Misfit |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Terminator |
| Found By | Brownstead, Hilary; Gustafson, Eric; Sayeed, Aatif |
| Year Found | 2010 |
| Location Found | Corona, CA USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.84125 N, 117.549055 W Map |
| Discovery Notes | Collected on 09/04/2010 at 4:40 pm.
Air temperature: 38ºC
Moist Soil, 4cm deep
An urban setting, 1 meter from a patch of grass. The sample location is adjacent to a wooden divider and artificial white stones. The sample was surrounded by tomato plants and 15 cm away from peppermint plants. The sample location is .6 meters from an apple tree. The sample area was in a vegetable garden of a single-family home (about 1.5 meters away from the foundation). The area is watered daily. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 6, 2019 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 181 |
| Sanger Finishing Reactions | 14 |
| Genome length (bp) | 76169 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques range in size from 0.5mm to 2.0mm in diameter. Plaques are turbid and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 144 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT522005 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
WUSL |
| Date of SEA Lysate Titering |
Nov 2, 2010 |
| Pitt Freezer Box# |
20 |
| Pitt Freezer Box Grid# |
27 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
