| Detailed Information for Phage MollyToe |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Molly Tobin |
| Year Found | 2025 |
| Location Found | Helena, MT United States |
| Finding Institution | Montana Technological University |
| Program | Montana Tech Bringing Research Into the Classroom |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 46.6 N, 112.05 W Map |
| Discovery Notes | Discovered in garden soil in Helena, Montana by a CR Anderson Middle School student named Molly Tobin. Molly Tobin's teacher was Jared Hunt. Exact location is unknown. |
| Naming Notes | I believe the phage is named after the student that found it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 20, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1174 |
| Genome length (bp) | 73178 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Montana Technological University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Phage has an isohedral head with a diameter of 56 nm and a tail with a length of 218 nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 136 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
