| Detailed Information for Phage Mosby |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | J. Bai, K. Dinh, and M. Huang |
| Year Found | 2012 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.073 N, 118.451 W Map |
| Discovery Notes | Isolated in MIMG 103AL (spring 2012) and annotated in MIMG 103BL (fall 2012) by Team How I Met Your Phage: Johnny Bai, Kimberly Dinh, and Melissa Huang
Instructor: Erin Sanders
TA (AL): Jovo Vijanderan
TA (BL): Will Villella
Curation of genome annotation results in winter 2013 by 199 students: Khristina Ipapo
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 22, 2013 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 55 |
| Genome length (bp) | 74533 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques with an average diameter of 3.6mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 141 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF493883 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2012UCLAmosbyJB |
| SEA Lysate Titer |
2.9x10^9 |
| Date of SEA Lysate Titering |
May 15, 2012 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
E6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
