| Detailed Information for Phage Murica | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Team Miley Virus |
| Year Found | 2013 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.07 N, 118.451 W Map |
| Discovery Notes | Phage isolation performed in Fall 2013 and genome annotation performed in Spring 2014 by Team Miley Virus: Kola Awe, Dami Oshinuga, and Apryl Stafford. Annotation quality checked by Lei Yeh, Edric Yoon, and Iger Ostreni. TA: Samantha Capati. Instructor: Dr. Jordan Moberg Parker. DNA preparation performed by Krisanavane Reddi (UCLA). Assembly performed by William Villella (UCLA). Moist soil collected next to a mossy tree, foot traffic and regular sprinklers on October 3, 2013. |
| Naming Notes | Slang for America. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 11, 2014 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 81 |
| Genome length (bp) | 77053 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to View244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Becksu Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka FairyPath Filch FireRed Flypotenuse FoghornLeghorn Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC StormChicken Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thimble Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Murica plaque assays contain both completely clear plaques in addition to plaques with clear centers and turbid edges. Average size (diameter in mm): 3.5mm. Range (in mm): 3mm-5mm |
| Morphotype | Siphoviridae |
| Number of Genes | 147 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | No |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919525 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 23 |
| Pitt Freezer Box Grid# | C6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
