Mycobacterium phage Murphy
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Detailed Information for Phage Murphy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMichael Anderson
Year Found2011
Location FoundColumbus, OH USA
Finding InstitutionThe Ohio State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.004213 N, 83.021904 W Map
Discovery NotesVery wet and muddy. On the bank of the Olentangy River, underneath Woody Hayes Bridge in Columbus Ohio. Taken from 1 inch under the surface. Shaded all day.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 24, 2012
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage170
Genome length (bp)76179
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes2-3mm in diameter. Whole in the center that fades out towards the edge.
MorphotypeSiphoviridae
Number of Genes146
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Murphy_1

Murphy_2

Murphy_3

Murphy_4

Murphy_5
(terminase small subunit)

Murphy_6

Murphy_7

Murphy_8
(terminase large subunit)

Murphy_9

Murphy_10
(portal protein)

Murphy_11
(capsid maturation protease)

Murphy_12
(major capsid protein)

Murphy_13

Murphy_14
(head-to-tail adaptor)

Murphy_15
(head-to-tail stopper)

Murphy_16

Murphy_17
(tail terminator)

Murphy_18
(major tail subunit)

Murphy_19

Murphy_20
(tail assembly chaperone)

Murphy_21
(tail assembly chaperone)

Murphy_22
(HNH endonuclease)

Murphy_23
(tape measure protein)

Murphy_24
(minor tail protein)

Murphy_25
(minor tail protein)

Murphy_26
(minor tail protein)

Murphy_27
(minor tail protein)

Murphy_28
(D-Ala-D-Ala carboxypeptidase)

Murphy_29

Murphy_30

Murphy_31

Murphy_32

Murphy_33
(lysin A)

Murphy_34
(holin)

Murphy_35

Murphy_36
(lysin B)

Murphy_37

Murphy_38
(NrdH-like protein)

Murphy_39
(Lsr2)

Murphy_40

Murphy_41

Murphy_42

Murphy_43

Murphy_44

Murphy_45

Murphy_46

Murphy_47

Murphy_48

Murphy_49

Murphy_50

Murphy_51

Murphy_52

Murphy_53

Murphy_54
(tyrosine integrase)

Murphy_55

Murphy_56

Murphy_57
(immunity repressor)

Murphy_58

Murphy_59

Murphy_60
(pentapeptide repeat domain protein)

Murphy_61

Murphy_62

Murphy_63

Murphy_64

Murphy_65

Murphy_66

Murphy_67

Murphy_68

Murphy_69
(ssDNA binding protein, ERF family)

Murphy_70

Murphy_71

Murphy_72

Murphy_73

Murphy_74

Murphy_75

Murphy_76

Murphy_77
(HNH endonuclease domain protein)

Murphy_78

Murphy_79

Murphy_80

Murphy_81
(DnaB-like helicase)

Murphy_82

Murphy_83
(WhiB family transcription factor)

Murphy_84

Murphy_85

Murphy_86

Murphy_87

Murphy_88
(polynucleotide kinase)

Murphy_89

Murphy_90

Murphy_91
(RNA ligase)

Murphy_92

Murphy_93
(WhiB)

Murphy_94

Murphy_95

Murphy_96

Murphy_97

Murphy_98

Murphy_99
(DNA methylase)

Murphy_100
(ssDNA binding protein)

Murphy_101
(ClpP-like protease)

Murphy_102

Murphy_103

Murphy_104

Murphy_105
(HNH homing endonuclease domain protein)

Murphy_106 — tRNA
(tRNA Arg (cct))

Murphy_107 — tRNA
(tRNA Gly (tcc))

Murphy_108

Murphy_109

Murphy_110

Murphy_111

Murphy_112
(DnaQ-like DNA polymerase III subunit)

Murphy_113

Murphy_114
(RecA-like DNA recombinase)

Murphy_115

Murphy_116

Murphy_117

Murphy_118

Murphy_119

Murphy_120

Murphy_121

Murphy_122

Murphy_123

Murphy_124

Murphy_125

Murphy_126

Murphy_127

Murphy_128

Murphy_129

Murphy_130

Murphy_131

Murphy_132

Murphy_133

Murphy_134

Murphy_135

Murphy_136

Murphy_137

Murphy_138

Murphy_139

Murphy_140

Murphy_141

Murphy_142

Murphy_143

Murphy_144

Murphy_145

Murphy_146

Murphy_147

Murphy_148
(HNH endonuclease domain protein)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKC748971
Refseq NumberNC_021305
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator OHSU
Pitt Freezer Box# 26
Pitt Freezer Box Grid# 71
Available Files
Plaque PictureDownload