| Detailed Information for Phage Nicholas |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lydia Bricker, Sierra Hobbs, and Michelle Lapak |
| Year Found | 2016 |
| Location Found | Gulf Breeze, FL USA |
| Finding Institution | University of West Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.357100 N, 87.163900 W Map |
| Naming Notes | We named our phage as a tribute to the great actor of our time, Nicolas Cage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1885 |
| Genome length (bp) | 70413 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 10 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140421 |
| Refseq Number | None yet |
| Published in Paper | Chung HM et al 2017, Genome Announcements: Complete Genome Sequences of Mycobacteriophages Clautastrophe, Kingsolomon, Krypton555, and Nicholas |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
43 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |