| Detailed Information for Phage OhShagHennessy |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caitlyn Cason |
| Year Found | 2019 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.2249 N, 80.4183 W Map |
| Discovery Notes | Found outside of O'Shaughnessy Hall, a dorm on Virginia Tech's campus. It was in an area well maintained but not routinely walked on by students. The area was not in close proximity to animals, large trees or bushes, or bodies of water. |
| Naming Notes | As it was found outside of O'Shaughnessy Hall I named my phage after a play on words of the dorm name. There is a popular Key and Peele skit called Substitute Teacher where the consistently mispronounce names, including O'Shaughnessy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1366 |
| Genome length (bp) | 72623 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGAACGGCC |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were small in size but incredibly clear. There were no halos on any plates or turbidity that was visible. The clear plaques with defined edges made harvesting them easier. |
| Morphotype | Siphoviridae |
| Number of Genes | 118 |
| Number of tRNAs | 7 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW435855 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
102 |
| Pitt Freezer Box Grid# |
B9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
