| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | James Kuan Broughton |
| Year Found | 2015 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 29.8607 S, 30.980314 E Map |
| Discovery Notes | Soil from a flower pot in my garden, it is watered regularly and receives a lot of sunlight; half red sand taken from surrounding garden, half compost; relatively dry; taken from surface layer. |
| Naming Notes | Name is my Gran's nickname. She taught me everything she knew about gardening when I was young, and since the soil is from a flower pot, I decided to name the phage after her. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 6, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1534 |
| Genome length (bp) | 75675 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Immune to phage CJWI (cluster E) |
| Other Cluster Members |
|
| Annotating Institution | University of Kwazulu-Natal |
| Annotation Status | In GenBank |
| Plaque Notes | Large plaques (~4.5-5mm diameter) develop after 2 days overnight incubation at 37 deg. celsius with a uniform circular morphology, and distinct halos. Halos only form after 2 days of incubation. After 1 overnight incubation at 37 deg. celsius, plaques are small ~1-2mm diameter and halos are absent. Plaques did not form after 3 days incubation at room temperature (at the time ~20 deg. celsius). |
| Morphotype | Siphoviridae |
| Number of Genes | 145 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK016502 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^10 PFU/ml |
| Date of SEA Lysate Titering |
Aug 7, 2015 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
