| Detailed Information for Phage Petra64142 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Codie Schaut |
| Year Found | 2013 |
| Location Found | Pullman, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.731889 N, 117.154194 W Map |
| Discovery Notes | Wet Top soil
Dirt mound in front of Gannon Hall
2.5cm. under the surface |
| Naming Notes | It came from the name of a character from a certain anime I was watching at this time that died, in my opinion, too soon. Thus I named my phage after her. The numbers represent the plagues that were labeled during the Streak Testing Protocol. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 22, 2015 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Pacific Biosciences Sequencing |
| Genome length (bp) | 75842 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| End Determination Notes | Similarity to related phages. |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington State University |
| Annotation Status | Unknown |
| Plaque Notes | halo style features
roughly 1.0-2.0mm in size
circular in dimension |
| Morphotype | Siphoviridae |
| Number of Genes | 143 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.0x10^9 pfu/ml |
| Date of SEA Lysate Titering |
Oct 22, 2013 |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
B3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
