The Actinobacteriophage Database

Mycobacterium phage Pharsalus

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Detailed Information for Phage Pharsalus
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Former names	Prophagus
Found By	Ali Ahmed
Year Found	2012
Location Found	Philadelphia, PA USA
Finding Institution	Saint Joseph's University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	39.971822 N, 75.197344 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 6, 2014
Sequencing Facility	NC State Genomic Sciences Laboratory
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	2161
Genome length (bp)	75779
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Becksu Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka FairyPath Filch FireRed Flypotenuse FoghornLeghorn Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC StormChicken Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thimble Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	142
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Pharsalus_1 Pharsalus_2 Pharsalus_3 Pharsalus_4 Pharsalus_5 Pharsalus_6 (terminase small subunit) Pharsalus_7 Pharsalus_8 Pharsalus_9 (terminase large subunit) Pharsalus_10 Pharsalus_11 (portal protein) Pharsalus_12 (capsid maturation protease) Pharsalus_13 (major capsid protein) Pharsalus_14 Pharsalus_15 (head-to-tail adaptor) Pharsalus_16 (head-to-tail stopper) Pharsalus_17 Pharsalus_18 (tail terminator) Pharsalus_19 (major tail protein) Pharsalus_20 Pharsalus_21 (tail assembly chaperone) Pharsalus_22 (tail assembly chaperone) Pharsalus_23 (tape measure protein) Pharsalus_24 (minor tail protein) Pharsalus_25 (minor tail protein) Pharsalus_26 (minor tail protein) Pharsalus_27 (minor tail protein) Pharsalus_28 (D-Ala-D-Ala carboxypeptidase) Pharsalus_29 Pharsalus_30 Pharsalus_31 (helix-turn-helix DNA binding domain protein) Pharsalus_32 Pharsalus_33 (lysin A) Pharsalus_34 (holin) Pharsalus_35 Pharsalus_36 (lysin B) Pharsalus_37 Pharsalus_38 (glutaredoxin) Pharsalus_39 (DNA bridging protein) Pharsalus_40 Pharsalus_41 Pharsalus_42 Pharsalus_43 Pharsalus_44 Pharsalus_45 Pharsalus_46 Pharsalus_47 Pharsalus_48 Pharsalus_49 Pharsalus_50 (tyrosine integrase) Pharsalus_51 Pharsalus_52 Pharsalus_53 (immunity repressor) Pharsalus_54 Pharsalus_55 Pharsalus_56 (pentapeptide repeat protein) Pharsalus_57 Pharsalus_58 Pharsalus_59 Pharsalus_60 Pharsalus_61 Pharsalus_62 Pharsalus_63 Pharsalus_64 Pharsalus_65 Pharsalus_66 (ssDNA binding protein, ERF family) Pharsalus_67 Pharsalus_68 Pharsalus_69 Pharsalus_70 Pharsalus_71 Pharsalus_72 Pharsalus_73 Pharsalus_74 (HNH endonuclease) Pharsalus_75 Pharsalus_76 Pharsalus_77 Pharsalus_78 (dsDNA helicase) Pharsalus_79 Pharsalus_80 (WhiB family transcription factor) Pharsalus_81 Pharsalus_82 Pharsalus_83 Pharsalus_84 Pharsalus_85 (polynucleotide kinase) Pharsalus_86 Pharsalus_87 Pharsalus_88 (RNA ligase) Pharsalus_89 (helix-turn-helix DNA binding domain protein) Pharsalus_90 (WhiB family transcription factor) Pharsalus_91 Pharsalus_92 Pharsalus_93 Pharsalus_94 Pharsalus_95 Pharsalus_96 (DNA methylase) Pharsalus_97 (ssDNA binding protein) Pharsalus_98 (ClpP-like protease) Pharsalus_99 Pharsalus_100 Pharsalus_101 Pharsalus_102 Pharsalus_103 (HNH endonuclease) Pharsalus_104 — tRNA (codon recognized: AGG) Pharsalus_105 — tRNA (codon recognized: GGA) Pharsalus_106 Pharsalus_107 Pharsalus_108 (hydrolase) Pharsalus_109 Pharsalus_110 (DnaQ-like DNA polymerase III subunit) Pharsalus_111 Pharsalus_112 Pharsalus_113 Pharsalus_114 Pharsalus_115 (RecA-like DNA recombinase) Pharsalus_116 Pharsalus_117 Pharsalus_118 Pharsalus_119 Pharsalus_120 Pharsalus_121 Pharsalus_122 Pharsalus_123 Pharsalus_124 Pharsalus_125 Pharsalus_126 Pharsalus_127 Pharsalus_128 Pharsalus_129 Pharsalus_130 Pharsalus_131 Pharsalus_132 Pharsalus_133 Pharsalus_134 Pharsalus_135 Pharsalus_136 Pharsalus_137 Pharsalus_138 Pharsalus_139 Pharsalus_140 Pharsalus_141 Pharsalus_142 Pharsalus_143 Pharsalus_144
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KX611831
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	39
Pitt Freezer Box Grid#	40
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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