| Detailed Information for Phage Pumpkin |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caitlin Peirce |
| Year Found | 2008 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.7697 N, 86.1664 W Map |
| Discovery Notes | From vegetable compost, clumpy moist soil. Depth 4 cm. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 23, 2009 |
| Sequencing Facility | Joint Genomics Institute |
| Shotgun Sequencing Method | Sanger |
| Sequencer Used | Sanger ABI 3730 |
| Read Type | Paired-end reads |
| Genome length (bp) | 74491 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 4 mm bulls-eye morphology after 24 hours of incubation at 37ºC. The center turbid circle has a diameter of 1-2 mm with a clear ring around it. |
| Morphotype | Siphoviridae |
| Number of Genes | 143 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | GQ303265 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2008HOPEpumpkinCEP |
| SEA Lysate Titer |
5.2e10 |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
