The Actinobacteriophage Database | Phage Quallification

Mycobacterium phage Quallification

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Detailed Information for Phage Quallification
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Allyson Qualley, Makenzie Cutillo
Year Found	2020
Location Found	Nahant, MA USA
Finding Institution	Merrimack College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	21°C
GPS Coordinates	42.426597 N, 70.923828 W Map
Naming Notes	Allyson and Makenzie both succeeded in isolating phage using the direct isolation method. Allyson's phage was lost during the amplification step, probably due to having to stay in the fridge too long during a COVID campus shutdown. Makenzie succeeded in amplifying hers and isolating its DNA. So, Makenzie contributed the phage, Allyson contributed the name, a play on her last name, Qualley.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 20, 2021
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	828
Genome length (bp)	75908
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Merrimack College
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	144
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Quallification_1 Quallification_2 Quallification_3 Quallification_4 Quallification_5 Quallification_6 (terminase small subunit) Quallification_7 Quallification_8 Quallification_9 Quallification_10 (terminase large subunit) Quallification_11 (RNA binding protein) Quallification_12 (portal protein) Quallification_13 (capsid maturation protease) Quallification_14 (major capsid protein) Quallification_15 Quallification_16 (head-to-tail adaptor) Quallification_17 (head-to-tail stopper) Quallification_18 Quallification_19 (tail terminator) Quallification_20 (major tail protein) Quallification_21 (tail assembly chaperone) Quallification_22 (tail assembly chaperone) Quallification_23 (tape measure protein) Quallification_24 (minor tail protein) Quallification_25 (minor tail protein) Quallification_26 (minor tail protein) Quallification_27 (minor tail protein) Quallification_28 (minor tail protein) Quallification_29 Quallification_30 Quallification_31 (helix-turn-helix DNA binding domain protein) Quallification_32 Quallification_33 (lysin A) Quallification_34 (holin) Quallification_35 Quallification_36 (lysin B) Quallification_37 Quallification_38 (NrdH-like glutaredoxin) Quallification_39 (Lsr2-like DNA bridging protein) Quallification_40 Quallification_41 Quallification_42 Quallification_43 Quallification_44 Quallification_45 Quallification_46 Quallification_47 Quallification_48 Quallification_49 Quallification_50 Quallification_51 Quallification_52 Quallification_53 (tyrosine integrase) Quallification_54 Quallification_55 Quallification_56 (immunity repressor) Quallification_57 Quallification_58 Quallification_59 (pentapeptide repeat protein) Quallification_60 Quallification_61 Quallification_62 Quallification_63 Quallification_64 Quallification_65 Quallification_66 Quallification_67 Quallification_68 (Cas4 family exonuclease) Quallification_69 Quallification_70 (ERF family ssDNA binding protein) Quallification_71 Quallification_72 Quallification_73 Quallification_74 Quallification_75 Quallification_76 Quallification_77 (DprA-like DNA processing chain A) Quallification_78 (HNH endonuclease) Quallification_79 Quallification_80 Quallification_81 Quallification_82 (DnaB-like dsDNA helicase) Quallification_83 Quallification_84 (WhiB family transcription factor) Quallification_85 Quallification_86 Quallification_87 (holliday junction resolvase) Quallification_88 Quallification_89 (polynucleotide kinase) Quallification_90 Quallification_91 Quallification_92 (RNA ligase) Quallification_93 (helix-turn-helix DNA binding domain protein) Quallification_94 (WhiB family transcription factor) Quallification_95 Quallification_96 Quallification_97 Quallification_98 Quallification_99 Quallification_100 (DNA methylase) Quallification_101 (ssDNA binding protein) Quallification_102 (ClpP-like protease) Quallification_103 Quallification_104 Quallification_105 Quallification_106 (helix-turn-helix DNA binding domain protein) Quallification_107 (HNH endonuclease) Quallification_108 — tRNA (tRNA-Arg(cct)) Quallification_109 — tRNA (tRNA-Gly(tcc)) Quallification_110 Quallification_111 Quallification_112 (deoxycytidylate deaminase) Quallification_113 Quallification_114 (DnaQ-like DNA polymerase III subunit) Quallification_115 Quallification_116 (RecA-like DNA recombinase) Quallification_117 Quallification_118 Quallification_119 Quallification_120 Quallification_121 Quallification_122 Quallification_123 Quallification_124 Quallification_125 Quallification_126 Quallification_127 Quallification_128 (helix-turn-helix DNA binding domain protein) Quallification_129 Quallification_130 Quallification_131 Quallification_132 Quallification_133 Quallification_134 Quallification_135 Quallification_136 Quallification_137 Quallification_138 Quallification_139 Quallification_140 Quallification_141 Quallification_142 Quallification_143 Quallification_144 Quallification_145 Quallification_146 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	ON970600
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	124
Pitt Freezer Box Grid#	G4
Available Files
Plaque Picture	Download
GenBank File for Phamerator	Download

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