| Detailed Information for Phage Silverleaf |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Raistland Valenzuela and Hari Kotturi |
| Year Found | 2017 |
| Location Found | Oklahoma City, OK USA |
| Finding Institution | University of Central Oklahoma |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.584167 N, 97.474167 W Map |
| Discovery Notes | The soil was collected underneath pine trees. The soil was moist. |
| Naming Notes | Address |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 1, 2017 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 371 |
| Genome length (bp) | 73210 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGAACGGCC |
| GC Content | 58.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Central Oklahoma |
| Annotation Status | In GenBank |
| Plaque Notes | The phage forms plaques clear plaques with 3mm diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 116 |
| Number of tRNAs | 11 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494092 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.0x10^10 PFU/ML |
| Date of SEA Lysate Titering |
Jun 28, 2017 |
| Pitt Freezer Box# |
72 |
| Pitt Freezer Box Grid# |
B11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
