The Actinobacteriophage Database

Mycobacterium phage Stark

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Detailed Information for Phage Stark
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	John Park
Year Found	2012
Location Found	Baltimore, MD USA
Finding Institution	Johns Hopkins University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	39.2986 N, 76.5946 W Map
Discovery Notes	The soil used was from a tomato plant patch. It was relatively wet. It also had various insects living in the patch.
Naming Notes	The name stark comes from one of my favorite tv series, the Game of Thrones.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Feb 6, 2013
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	140
Sanger Finishing Reactions	12
Genome length (bp)	74731
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	University of Pittsburgh
Annotation Status	In GenBank
Plaque Notes	Relatively small, Fuzzy on the outer areas of the plaque. about 1.5 mm diameter.
Morphotype	Siphoviridae
Number of Genes	141
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Stark_1 Stark_2 Stark_3 Stark_4 Stark_5 Stark_6 (terminase small subunit) Stark_7 Stark_8 Stark_9 (terminase large subunit) Stark_10 Stark_11 (portal protein) Stark_12 (capsid maturation protease) Stark_13 (major capsid protein) Stark_14 Stark_15 (head-to-tail adaptor) Stark_16 (head-to-tail stopper) Stark_17 Stark_18 (tail terminator) Stark_19 (major tail protein) Stark_20 Stark_21 (tail assembly chaperone) Stark_22 (tail assembly chaperone) Stark_23 (tape measure protein) Stark_24 (minor tail protein) Stark_25 (minor tail protein) Stark_26 (minor tail protein) Stark_27 (minor tail protein) Stark_28 (minor tail protein) Stark_29 Stark_30 Stark_31 (helix-turn-helix DNA binding domain protein) Stark_32 Stark_33 (lysin A) Stark_34 (holin) Stark_35 Stark_36 (lysin B) Stark_37 Stark_38 (NrdH-like glutaredoxin) Stark_39 (Lsr2-like DNA bridging protein) Stark_40 Stark_41 Stark_42 Stark_43 Stark_44 Stark_45 Stark_46 Stark_47 Stark_48 Stark_49 Stark_50 Stark_51 Stark_52 Stark_53 (tyrosine integrase) Stark_54 Stark_55 Stark_56 (immunity repressor) Stark_57 Stark_58 Stark_59 (pentapeptide repeat protein) Stark_60 Stark_61 Stark_62 Stark_63 Stark_64 Stark_65 Stark_66 Stark_67 Stark_68 (exonuclease) Stark_69 Stark_70 (ERF family ssDNA binding protein) Stark_71 Stark_72 Stark_73 Stark_74 Stark_75 Stark_76 Stark_77 (DprA-like DNA processing chain A) Stark_78 (HNH endonuclease) Stark_79 Stark_80 Stark_81 Stark_82 (DnaB-like DNA helicase) Stark_83 Stark_84 (WhiB family transcription factor) Stark_85 Stark_86 Stark_87 (holliday junction resolvase) Stark_88 Stark_89 (polynucleotide kinase) Stark_90 Stark_91 Stark_92 Stark_93 (RNA ligase) Stark_94 (helix-turn-helix DNA binding domain protein) Stark_95 (WhiB family transcription factor) Stark_96 Stark_97 Stark_98 Stark_99 Stark_100 (DNA methylase) Stark_101 (ssDNA binding protein) Stark_102 (ClpP-like protease) Stark_103 Stark_104 Stark_105 (excise) Stark_106 (HNH endonuclease) Stark_107 — tRNA (tRNA-Arg(cct)) Stark_108 — tRNA (tRNA-Gly(tcc)) Stark_109 Stark_110 Stark_111 (deoxycytidylate deaminase) Stark_112 Stark_113 (DnaQ-like DNA polymerase III subunit) Stark_114 Stark_115 (RecA-like DNA recombinase) Stark_116 Stark_117 Stark_118 Stark_119 Stark_120 Stark_121 Stark_122 Stark_123 Stark_124 Stark_125 Stark_126 (helix-turn-helix DNA binding domain protein) Stark_127 Stark_128 Stark_129 Stark_130 Stark_131 Stark_132 Stark_133 Stark_134 Stark_135 Stark_136 Stark_137 Stark_138 Stark_139 Stark_140 Stark_141 Stark_142 Stark_143 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN586019
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	13
Pitt Freezer Box Grid#	C9
Available Files
Plaque Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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