Mycobacterium phage TBrady12
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Detailed Information for Phage TBrady12
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesTomBrady
Found ByConnor Gilson, Brian Elsemore
Year Found2016
Location FoundOrono, ME USA
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates44.895600 N, 68.673600 W Map
Discovery NotesSunny, 78º F,
4 inches deep,
Sample collected from fertilized flower pot,
Naming NotesWe named our phage after the greatest quarterback to ever live. We thought this name was appropriate because our phage is the greatest phage to ever exist.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1703
Genome length (bp)75359
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
Plaque NotesClear plaques, with slight turbid "halos".
MorphotypeSiphoviridae
Number of Genes149
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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TBrady12_1

TBrady12_2

TBrady12_3

TBrady12_4

TBrady12_5

TBrady12_6
(terminase small subunit)

TBrady12_7

TBrady12_8

TBrady12_9

TBrady12_10
(terminase large subunit)

TBrady12_11

TBrady12_12
(portal protein)

TBrady12_13
(capsid maturation protease)

TBrady12_14
(major capsid protein)

TBrady12_15

TBrady12_16
(head-to-tail adaptor)

TBrady12_17
(head-to-tail stopper)

TBrady12_18

TBrady12_19
(tail terminator)

TBrady12_20
(major tail protein)

TBrady12_21

TBrady12_22
(tail assembly chaperone)

TBrady12_23
(tail assembly chaperone)

TBrady12_24
(tape measure protein)

TBrady12_25
(minor tail protein)

TBrady12_26
(minor tail protein)

TBrady12_27
(minor tail protein)

TBrady12_28
(minor tail protein)

TBrady12_29
(D-Ala-D-Ala carboxypeptidase)

TBrady12_30
(minor tail protein)

TBrady12_31

TBrady12_32
(helix-turn-helix DNA binding domain protein)

TBrady12_33

TBrady12_34
(lysin A)

TBrady12_35
(holin)

TBrady12_36

TBrady12_37
(lysin B)

TBrady12_38

TBrady12_39
(NrdH-like glutaredoxin)

TBrady12_40
(Lsr2-like DNA bridging protein)

TBrady12_41

TBrady12_42

TBrady12_43

TBrady12_44

TBrady12_45

TBrady12_46

TBrady12_47

TBrady12_48

TBrady12_49

TBrady12_50

TBrady12_51

TBrady12_52

TBrady12_53

TBrady12_54

TBrady12_55
(tyrosine integrase)

TBrady12_56

TBrady12_57

TBrady12_58
(immunity repressor)

TBrady12_59

TBrady12_60

TBrady12_61
(pentapeptide repeat protein)

TBrady12_62

TBrady12_63

TBrady12_64

TBrady12_65

TBrady12_66

TBrady12_67

TBrady12_68

TBrady12_69

TBrady12_70
(exonuclease)

TBrady12_71

TBrady12_72
(ERF family ssDNA binding protein)

TBrady12_73

TBrady12_74

TBrady12_75

TBrady12_76

TBrady12_77

TBrady12_78

TBrady12_79
(DprA-like DNA processing chain A)

TBrady12_80
(HNH endonuclease)

TBrady12_81

TBrady12_82

TBrady12_83

TBrady12_84
(DnaB-like dsDNA helicase)

TBrady12_85

TBrady12_86
(WhiB family transcription factor)

TBrady12_87

TBrady12_88

TBrady12_89
(holliday junction resolvase)

TBrady12_90

TBrady12_91
(polynucleotide kinase)

TBrady12_92

TBrady12_93

TBrady12_94
(RNA ligase)

TBrady12_95
(helix-turn-helix DNA binding domain protein)

TBrady12_96
(WhiB family transcription factor)

TBrady12_97

TBrady12_98

TBrady12_99

TBrady12_100

TBrady12_101

TBrady12_102
(endonuclease VII)

TBrady12_103
(ssDNA binding protein)

TBrady12_104
(ClpP-like protease)

TBrady12_105

TBrady12_106

TBrady12_107

TBrady12_108
(helix-turn-helix DNA binding domain protein)

TBrady12_109
(HNH endonuclease)

TBrady12_110 — tRNA
(tRNA-Arg(cct))

TBrady12_111 — tRNA
(tRNA-Gly(tcc))

TBrady12_112

TBrady12_113

TBrady12_114
(HNH endonuclease)

TBrady12_115
(glycosyltransferase)

TBrady12_116

TBrady12_117
(DnaQ-like DNA polymerase III subunit)

TBrady12_118

TBrady12_119
(RecA-like DNA recombinase)

TBrady12_120

TBrady12_121

TBrady12_122

TBrady12_123

TBrady12_124

TBrady12_125

TBrady12_126

TBrady12_127

TBrady12_128

TBrady12_129

TBrady12_130

TBrady12_131
(helix-turn-helix DNA binding domain protein)

TBrady12_132

TBrady12_133

TBrady12_134

TBrady12_135

TBrady12_136

TBrady12_137

TBrady12_138

TBrady12_139

TBrady12_140

TBrady12_141

TBrady12_142

TBrady12_143

TBrady12_144

TBrady12_145

TBrady12_146

TBrady12_147

TBrady12_148

TBrady12_149

TBrady12_150

TBrady12_151
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH536829
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 44
Pitt Freezer Box Grid# G3
Available Files
EM PictureDownload
GenBank File for PhameratorDownload