| Detailed Information for Phage Teaspoon |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Elise DeRosby and Evie Schlett |
| Year Found | 2023 |
| Location Found | Orono, ME United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 44.89681 N, 68.66881 W Map |
| Discovery Notes | Phage took multiple cycles of soil sampling to isolate, and once isolated, needs multiple enrichments to amplify. |
| Naming Notes | Named teaspoon because it is tiny. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 444 |
| Genome length (bp) | 70942 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | In GenBank |
| Plaque Notes | Inconsistent plaque morphology. All plaques are turbid, ranging from .5mm-.8mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 128 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978847 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
186 |
| Pitt Freezer Box Grid# |
G8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
