| Detailed Information for Phage Terminus |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Camille Ocampo |
| Year Found | 2014 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.0675 N, 118.45444 W Map |
| Discovery Notes | Phage Terminus was isolated from soil collected from a potted palm tree plant in Los Angeles, CA. Phage Terminus was isolated, characterized and annotated by teams BLAST from the Phage (Jenny Kan, Kevin Louie, Camille Ocampo) and Hakuna Maphagea (Tracy De los Santos, Benjamin Lopez). Instructor: Giorgia Pirino. TA: Rocky Ng. Annotation was quality checked by Stella Joh. |
| Naming Notes | Terminus is the name of the Roman god of boundaries. It also means the "end of the line" for transportation routes. In the popular TV show The Walking Dead, Terminus is the name of a survivor camp of cannibals housed in a railway terminal station. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 16, 2015 |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 10582 |
| Genome length (bp) | 76169 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | No evidence of lysogeny due to clear plaques and lack of integrase gene |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were mostly clear, and ranged in diameter from 2-4 mm, averaging 3 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 147 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919535 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
E1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
