| Detailed Information for Phage Thimble |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Catherine Beazley, McKenzie Shaw |
| Year Found | 2024 |
| Location Found | Hampden, ME United States of America |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.7047 N, 68.91632 W Map |
| Discovery Notes | Taken from garden soil in a strawberry garden, about an inch below the surface, under dry conditions. |
| Naming Notes | Picked this name due to the small size of the plaques. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 949 |
| Genome length (bp) | 71466 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Honors College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Morphotype | Siphoviridae |
| Number of Genes | 134 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
