| Detailed Information for Phage Thresher |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cydne Kitchens |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.008335 N, 86.376597 W Map |
| Discovery Notes | The soil sample was taken from the side of the road next to a branch of Fruit of the Loom. |
| Naming Notes | It was named after my favorite species of shark. This species of shark is known for having an exceptionally long caudal fin, similar to the long tail of a siphoviridae. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 17, 2021 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 185 |
| Genome length (bp) | 76192 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | This phage produced clear plaques. This made it easier to see, work with, and count. |
| Morphotype | Siphoviridae |
| Number of Genes | 146 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ622158 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUtresherCK |
| SEA Lysate Titer |
1.3 x 10^10 pfu/mL |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
D9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
