The Actinobacteriophage Database

Mycobacterium phage Toto

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Detailed Information for Phage Toto
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Lucia Barker
Year Found	2008
Location Found	Ashburn, VA USA
Finding Institution	Howard Hughes Medical Institute
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	39.0702 N, 77.4639 W Map
Discovery Notes	From soil from JFRC Roof - under a tree
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 31, 2011
Genome length (bp)	75933
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Variable morphology - ~2mm/irregularly shaped turbid plaques, roundish clear plaques after 24 hour incubation at 37°C on 7H10 plates.
Morphotype	Siphoviridae
Number of Genes	140
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Toto_1 Toto_2 Toto_3 Toto_4 Toto_5 Toto_6 (terminase small subunit) Toto_7 Toto_8 Toto_9 (terminase large subunit) Toto_10 Toto_11 (portal protein) Toto_12 (capsid maturation protease) Toto_13 (major capsid protein) Toto_14 Toto_15 (head-to-tail adaptor) Toto_16 (head-to-tail stopper) Toto_17 Toto_18 (tail terminator) Toto_19 (major tail subunit) Toto_20 (tail assembly chaperone) Toto_21 (tail assembly chaperone) Toto_22 (tape measure protein) Toto_23 (minor tail protein) Toto_24 (minor tail protein) Toto_25 (minor tail protein) Toto_26 (minor tail protein) Toto_27 (minor tail protein) Toto_28 Toto_29 Toto_30 Toto_31 Toto_32 (lysin A) Toto_33 (holin) Toto_34 Toto_35 (lysin B) Toto_36 Toto_37 (NrdH) Toto_38 (HNH endonuclease domain protein) Toto_39 (Lsr2) Toto_40 Toto_41 Toto_42 Toto_43 Toto_44 Toto_45 Toto_46 Toto_47 Toto_48 Toto_49 Toto_50 Toto_51 (tyrosine integrase) Toto_52 Toto_53 Toto_54 Toto_55 Toto_56 Toto_57 Toto_58 Toto_59 Toto_60 Toto_61 Toto_62 Toto_63 Toto_64 Toto_65 Toto_66 Toto_67 (ssDNA binding protein, ERF family) Toto_68 Toto_69 Toto_70 Toto_71 Toto_72 Toto_73 Toto_74 Toto_75 Toto_76 Toto_77 Toto_78 Toto_79 (DnaB) Toto_80 Toto_81 (WhiB family transcription factor) Toto_82 Toto_83 Toto_84 Toto_85 Toto_86 (polynucleotide kinase) Toto_87 Toto_88 Toto_89 (RNA ligase) Toto_90 Toto_91 (WhiB family transcription factor) Toto_92 Toto_93 Toto_94 Toto_95 Toto_96 Toto_97 (methyltransferase) Toto_98 (single-stranded DNA-binding protein) Toto_99 (ClpP-like protease) Toto_100 Toto_101 Toto_102 Toto_103 Toto_104 Toto_105 — tRNA (tRNA-Arg (cct)) Toto_106 — tRNA (tRNA-Gly (tcc)) Toto_107 Toto_108 Toto_109 Toto_110 Toto_111 Toto_112 (DnaQ-like DNA polymerase III subunit) Toto_113 Toto_114 (RecA-like DNA recombinase) Toto_115 Toto_116 Toto_117 Toto_118 Toto_119 Toto_120 Toto_121 Toto_122 Toto_123 Toto_124 Toto_125 Toto_126 Toto_127 (ParB-like nuclease domain protein) Toto_128 Toto_129 Toto_130 Toto_131 Toto_132 Toto_133 Toto_134 Toto_135 Toto_136 Toto_137 Toto_138 Toto_139 Toto_140 Toto_141 Toto_142
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	JN006061
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2008HHMITotoKWB
SEA Lysate Titer	3.7e9
Pitt Freezer Box#	3
Pitt Freezer Box Grid#	B9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download

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