| Detailed Information for Phage Ukulele |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Maryanne LaFollette and Matthew Sheltra |
| Year Found | 2011 |
| Location Found | Old Orchard Beach, ME USA |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 43.517800 N, 70.377300 W Map |
| Discovery Notes | Soil sample was found in a flower garden ~2 cm deep. Temperature was approximately 69?, and soil was slightly damp. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 31, 2012 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 133 |
| Genome length (bp) | 75114 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque morphology ranges from ~0.5 mm to ~2.0 mm, and is slightly turbid. |
| Morphotype | Siphoviridae |
| Number of Genes | 141 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT373978 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
13 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
