Detailed Information for Phage Wigglewiggle |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | Swigilywiggily |
Found By | Arianna Martin |
Year Found | 2018 |
Location Found | Blacksburg, VA USA |
Finding Institution | Virginia Tech |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 37.224 N, 80.4232 W Map |
Discovery Notes | The phage was found near Fralin Hall approximately 10 ft from the building. |
Naming Notes | The name of my phage was created because I imagined it would be really wiggly when viewed because my phage has a very long tail length. This tail would cause it to squirm and wiggle. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Nov 30, 2018 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 1042 |
Genome length (bp) | 76414 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | TCGATCAGCC |
GC Content | 59.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | L |
Subcluster | L2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Virginia Tech |
Annotation Status | In GenBank |
Plaque Notes | The plaques are polymorphic. After the third purification some of the plaques were very small while others were 3 times the size of the small ones. The plaques were dark and turbid. |
Morphotype | Siphoviridae |
Number of Genes | 134 |
Number of tRNAs | 11 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK937600 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
80 |
Pitt Freezer Box Grid# |
D9 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |