| Detailed Information for Phage Wigglewiggle |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Swigilywiggily |
| Found By | Arianna Martin |
| Year Found | 2018 |
| Location Found | Blacksburg, VA USA |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.224 N, 80.4232 W Map |
| Discovery Notes | The phage was found near Fralin Hall approximately 10 ft from the building. |
| Naming Notes | The name of my phage was created because I imagined it would be really wiggly when viewed because my phage has a very long tail length. This tail would cause it to squirm and wiggle. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1042 |
| Genome length (bp) | 76414 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are polymorphic. After the third purification some of the plaques were very small while others were 3 times the size of the small ones. The plaques were dark and turbid. |
| Morphotype | Siphoviridae |
| Number of Genes | 134 |
| Number of tRNAs | 11 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK937600 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
80 |
| Pitt Freezer Box Grid# |
D9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
