Mycobacterium phage YassJohnny
YassJohnny Plaque Picture
YassJohnny EM Picture
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Detailed Information for Phage YassJohnny
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesYasssJohnny
Found ByTeams Phage and Chill & Trap Phages
Year Found2015
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.065618 N, 118.454008 W Map
Discovery NotesSoil collected by Johnny Pham. Phage isolated, purified and characterized by Teams Phage and Chill & Trap Phages.

Team Phage and Chill:
Johnny Pham
Sejal Mehta
Judy Vo
Veronica Gonzalez


Team: Trap Phages
Emily Chiu
Annika Taber
Ruoxi Zhang
Jennifer Briones

TA: Fasih Ahsan
Instructor: Giorgia Pirino

Sample description:
-Sample was taken near a root of plant in the garden of an apartment complex in an Urban area. Air temperature:87° F
Depth from which sample was taken: 5 cm
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage913
Genome length (bp)73697
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Sequencing NotesThere is a polymorphism in this genome. At position 28117, about 45% of the reads have a T instead of the currently called consensus C.
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes
MorphotypeSiphoviridae
Number of Genes139
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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YassJohnny_1

YassJohnny_2

YassJohnny_3

YassJohnny_4

YassJohnny_5

YassJohnny_6
(terminase small subunit)

YassJohnny_7

YassJohnny_8

YassJohnny_9
(terminase large subunit)

YassJohnny_10

YassJohnny_11
(portal protein)

YassJohnny_12
(capsid maturation protease)

YassJohnny_13
(major capsid protein)

YassJohnny_14

YassJohnny_15
(head-to-tail adaptor)

YassJohnny_16
(head-to-tail stopper)

YassJohnny_17

YassJohnny_18
(tail terminator)

YassJohnny_19
(major tail protein)

YassJohnny_20
(tail assembly chaperone)

YassJohnny_21
(tail assembly chaperone)

YassJohnny_22
(HNH endonuclease)

YassJohnny_23
(tape measure protein)

YassJohnny_24
(minor tail protein)

YassJohnny_25
(minor tail protein)

YassJohnny_26
(minor tail protein)

YassJohnny_27
(minor tail protein)

YassJohnny_28
(minor tail protein)

YassJohnny_29
(minor tail protein)

YassJohnny_30

YassJohnny_31
(helix-turn-helix DNA binding domain protein)

YassJohnny_32

YassJohnny_33
(lysin A)

YassJohnny_34
(holin)

YassJohnny_35

YassJohnny_36
(lysin B)

YassJohnny_37

YassJohnny_38
(glutaredoxin)

YassJohnny_39
(DNA bridging protein)

YassJohnny_40

YassJohnny_41

YassJohnny_42

YassJohnny_43

YassJohnny_44

YassJohnny_45

YassJohnny_46

YassJohnny_47
(membrane domain protein)

YassJohnny_48
(tyrosine integrase)

YassJohnny_49

YassJohnny_50

YassJohnny_51
(immunity repressor)

YassJohnny_52

YassJohnny_53

YassJohnny_54
(pentapeptide repeat protein)

YassJohnny_55

YassJohnny_56

YassJohnny_57

YassJohnny_58

YassJohnny_59

YassJohnny_60

YassJohnny_61

YassJohnny_62

YassJohnny_63

YassJohnny_64

YassJohnny_65

YassJohnny_66
(ERF family ssDNA binding protein)

YassJohnny_67

YassJohnny_68

YassJohnny_69

YassJohnny_70

YassJohnny_71

YassJohnny_72

YassJohnny_73

YassJohnny_74
(HNH endonuclease)

YassJohnny_75

YassJohnny_76

YassJohnny_77

YassJohnny_78
(dsDNA helicase)

YassJohnny_79

YassJohnny_80
(WhiB family transcription factor)

YassJohnny_81

YassJohnny_82

YassJohnny_83
(holliday junction resolvase)

YassJohnny_84

YassJohnny_85
(polynucleotide kinase)

YassJohnny_86

YassJohnny_87

YassJohnny_88
(RNA ligase)

YassJohnny_89
(helix-turn-helix DNA binding domain protein)

YassJohnny_90
(WhiB family transcription factor)

YassJohnny_91

YassJohnny_92

YassJohnny_93

YassJohnny_94

YassJohnny_95

YassJohnny_96
(DNA methylase)

YassJohnny_97
(ssDNA binding protein)

YassJohnny_98
(ClpP-like protease)

YassJohnny_99

YassJohnny_100

YassJohnny_101

YassJohnny_102
(helix-turn-helix DNA binding domain protein)

YassJohnny_103
(HNH endonuclease)

YassJohnny_104 — tRNA
(tRNA-Arg (cct))

YassJohnny_105 — tRNA
(tRNA-Gly (tcc))

YassJohnny_106

YassJohnny_107

YassJohnny_108

YassJohnny_109

YassJohnny_110
(DnaQ-like DNA polymerase III subunit)

YassJohnny_111

YassJohnny_112
(RecA-like DNA recombinase)

YassJohnny_113

YassJohnny_114

YassJohnny_115

YassJohnny_116

YassJohnny_117

YassJohnny_118

YassJohnny_119

YassJohnny_120

YassJohnny_121

YassJohnny_122

YassJohnny_123

YassJohnny_124
(helix-turn-helix DNA binding domain protein)

YassJohnny_125

YassJohnny_126

YassJohnny_127

YassJohnny_128

YassJohnny_129

YassJohnny_130

YassJohnny_131

YassJohnny_132

YassJohnny_133

YassJohnny_134

YassJohnny_135

YassJohnny_136

YassJohnny_137

YassJohnny_138

YassJohnny_139

YassJohnny_140

YassJohnny_141

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919541
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 23
Pitt Freezer Box Grid# E10
Available Files
GenBank File for PhameratorDownload