| Detailed Information for Phage YassJohnny |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | YasssJohnny |
| Found By | Teams Phage and Chill & Trap Phages |
| Year Found | 2015 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.065618 N, 118.454008 W Map |
| Discovery Notes | Soil collected by Johnny Pham. Phage isolated, purified and characterized by Teams Phage and Chill & Trap Phages.
Team Phage and Chill:
Johnny Pham
Sejal Mehta
Judy Vo
Veronica Gonzalez
Team: Trap Phages
Emily Chiu
Annika Taber
Ruoxi Zhang
Jennifer Briones
TA: Fasih Ahsan
Instructor: Giorgia Pirino
Sample description:
-Sample was taken near a root of plant in the garden of an apartment complex in an Urban area. Air temperature:87° F
Depth from which sample was taken: 5 cm
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 913 |
| Genome length (bp) | 73697 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Sequencing Notes | There is a polymorphism in this genome. At position 28117, about 45% of the reads have a T instead of the currently called consensus C. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes |
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| Morphotype | Siphoviridae |
| Number of Genes | 139 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919541 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
E10 |
| Available Files |
| GenBank File for Phamerator | Download |