| Detailed Information for Phage Youngblood |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Team Bad Assay |
| Year Found | 2014 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.075 N, 118.451 W Map |
| Discovery Notes | Phage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Bad Assay: Arash Amighi, Chaya Patel, Yvonne Lam, Steven Youngblood. Annotation quality checked by Edric Yoon and Lei Yeh. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).
Sample description: Dry, dark brown, grainy soil collected at a 6cm depth by Yvonne Lam on April 4, 2014. Area is urban and flat. The location is near trees and foot traffic. |
| Naming Notes | Named after team member Steven Youngblood. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 38 |
| Genome length (bp) | 75896 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Evidence for lysogeny: bullseye plaque morphology, and genome contains integrase gene. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology had a bullseye or turbid morphology depending on the dilution. The plaques were circular ranging from 0.2 to 0.5 cm with the average size of 3.37 mm and standard deviation of 0.43 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 148 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG099953 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
