| Detailed Information for Phage Zameen |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Duplicates of this Phage | Azaula98 |
| Found By | Jaspreet Singh |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.44189 N, 79.9557 W Map |
| Discovery Notes | Collected outside Barco Building in light brown (softball diamond) colored dirt next to a big maple tree. |
| Naming Notes | My family originates from Punjab, and so the name I chose, Zameen, is Punjabi for "earth" or "land." I chose this name because the soil sample came, literally, from the Earth. I also chose this name because it has a special personal significance to me - I am the first women in my family to pursue medicine and science research professionally. I wanted to chose a name that sounded pretty - (Phonetically it is ZUH - MEAN) but also showed how proud I am of my heritage and the opportunities I have today. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 346 |
| Genome length (bp) | 50337 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Lysogen testing revealed that Zameen is likely a temperate phage, as it's plaques have hazy, rather than clear, edges. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques on Zameen are round and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK878898 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
91 |
| Pitt Freezer Box Grid# |
E2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
