| Detailed Information for Phage xkcd |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jessica Ruby |
| Year Found | 2008 |
| Location Found | Santa Cruz, CA USA |
| Finding Institution | University of California, Santa Cruz |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.983233 N, 122.061133 W Map |
| Discovery Notes | Taken from potted soil at the UCSC Arboretum, depth 0.5-1 inches, 65°F, low, well-protected, shady, moist |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 16, 2015 |
| Sequencing Facility | University of California, Santa Cruz |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 22 |
| Sanger Finishing Reactions | 4 |
| Genome length (bp) | 76915 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Medium-sized, slightly under 1 cm on average for lower concentrations. At higher concentrations, shrunk to ~4mm.
Round, slightly turbid, occasional resistant bacterial colonies visible in plaques
24-hour incubations at 37°C |
| Morphotype | Siphoviridae |
| Number of Genes | 149 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH590587 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2008UCSCxkcdJAR |
| SEA Lysate Titer |
1.8x10^10 |
| Pitt Freezer Box# |
6 |
| Pitt Freezer Box Grid# |
68 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
