| Detailed Information for Phage Acquire49 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tyler Burton |
| Year Found | 2016 |
| Location Found | Nancy, KY USA |
| Finding Institution | Somerset Community College |
| Program | Kentucky Biomedical Infrastructure Network Small Genomes Discovery Program |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.082778 N, 84.101389 W Map |
| Discovery Notes | Temperature: around 30 degrees F or below.
Date: February 15, 2016
Climate: It was snowy, damp, and cold.
Characteristics of soil: It was damp and the color of the soil was dark, but the soil had a soft texture to it.
From: Same enrichment as PhantomSKS |
| Naming Notes | To be honest I sat down with my girlfriend and told her to name off random letters and numbers with the first character being capital. She came up with A3cq49. For the Database it was named Acquire49. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 23, 2016 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 407 |
| Genome length (bp) | 73639 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGAACGGCC |
| GC Content | 58.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Somerset Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, very round, lytic in nature. Approximately 2mm in diameter.
|
| Morphotype | Siphoviridae |
| Number of Genes | 123 |
| Number of tRNAs | 9 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK279905 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.8x10^10 pfu/mL |
| Date of SEA Lysate Titering |
Apr 26, 2016 |
| Pitt Freezer Box# |
28 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
