Mycobacterium phage Appletree2
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Detailed Information for Phage Appletree2
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTina Slowan-Pomeroy
Year Found2014
Location FoundReno, NV USA
Finding InstitutionTruckee Meadows Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates39.3825 N, 119.7416 W Map
Discovery NotesFrom soil underneath an apple tree in a residential neighborhood.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 18, 2014
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1006
Genome length (bp)73808
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesDiameter of plaques: 0.85mm
MorphotypeSiphoviridae
Number of Genes125
Number of tRNAs8
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Appletree2_1

Appletree2_2
(terminase small subunit)

Appletree2_3

Appletree2_4
(terminase large subunit)

Appletree2_5
(portal protein)

Appletree2_6
(MuF-like minor capsid protein)

Appletree2_7
(scaffolding protein)

Appletree2_8
(major capsid protein)

Appletree2_9
(head-to-tail adaptor)

Appletree2_10
(head-to-tail stopper)

Appletree2_11

Appletree2_12
(tail terminator)

Appletree2_13
(major tail protein)

Appletree2_14
(tail assembly chaperone)

Appletree2_15
(tail assembly chaperone)

Appletree2_16
(tape measure protein)

Appletree2_17
(minor tail protein)

Appletree2_18
(minor tail protein)

Appletree2_19
(minor tail protein)

Appletree2_20
(minor tail protein)

Appletree2_21
(minor tail protein)

Appletree2_22

Appletree2_23

Appletree2_24

Appletree2_25
(lysin A)

Appletree2_26
(lysin B)

Appletree2_27

Appletree2_28

Appletree2_29

Appletree2_30
(NrdH-like glutaredoxin)

Appletree2_31

Appletree2_32
(nucleotidyltransferase)

Appletree2_33
(Lsr2-like DNA bridging protein)

Appletree2_34

Appletree2_35
(tyrosine integrase)

Appletree2_36

Appletree2_37

Appletree2_38
(Cro protein)

Appletree2_39
(excise)

Appletree2_40

Appletree2_41

Appletree2_42

Appletree2_43

Appletree2_44

Appletree2_45
(esterase)

Appletree2_46

Appletree2_47

Appletree2_48

Appletree2_49

Appletree2_50

Appletree2_51

Appletree2_52
(Ro-like RNA binding protein)

Appletree2_53

Appletree2_54

Appletree2_55

Appletree2_56

Appletree2_57

Appletree2_58

Appletree2_59
(Cas4 family exonuclease)

Appletree2_60

Appletree2_61
(ERF family ssDNA binding protein)

Appletree2_62

Appletree2_63

Appletree2_64

Appletree2_65

Appletree2_66

Appletree2_67
(DnaB-like dsDNA helicase)

Appletree2_68
(WhiB family transcription factor)

Appletree2_69

Appletree2_70

Appletree2_71
(phosphoribosyl pyrophosphate transferase)

Appletree2_72
(nicotinate ribosyltransferase)

Appletree2_73

Appletree2_74
(polynucleotide kinase)

Appletree2_75

Appletree2_76

Appletree2_77
(WhiB family transcription factor)

Appletree2_78
(WhiB family transcription factor)

Appletree2_79

Appletree2_80

Appletree2_81
(ssDNA-binding protein)

Appletree2_82

Appletree2_83

Appletree2_84

Appletree2_85
(RNA ligase)

Appletree2_86

Appletree2_87

Appletree2_88

Appletree2_89

Appletree2_90
(HNH endonuclease)

Appletree2_91

Appletree2_92

Appletree2_93
(DnaQ-like DNA polymerase III subunit)

Appletree2_94

Appletree2_95

Appletree2_96

Appletree2_97

Appletree2_98

Appletree2_99

Appletree2_100

Appletree2_101 — tRNA

Appletree2_102 — tRNA

Appletree2_103

Appletree2_104 — tRNA

Appletree2_105 — tRNA

Appletree2_106 — tRNA

Appletree2_107

Appletree2_108

Appletree2_109 — tRNA

Appletree2_110

Appletree2_111

Appletree2_112 — tRNA

Appletree2_113

Appletree2_114 — tRNA

Appletree2_115

Appletree2_116

Appletree2_117

Appletree2_118

Appletree2_119

Appletree2_120

Appletree2_121

Appletree2_122

Appletree2_123

Appletree2_124

Appletree2_125

Appletree2_126

Appletree2_127

Appletree2_128
(AAA-ATPase)

Appletree2_129

Appletree2_130

Appletree2_131

Appletree2_132

Appletree2_133

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF185726
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 15
Pitt Freezer Box Grid# C7
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload