The Actinobacteriophage Database

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Detailed Information for Phage Breezona
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Breeze Victor
Year Found	2011
Location Found	Brockton, MA USA
Finding Institution	Bucknell University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	42.063572 N, 71.022925 W Map
Discovery Notes	Loam soil, 3 inches deep, 83 degrees Farenheit.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 31, 2012
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	160
Genome length (bp)	76652
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	TCGATCAGCC
GC Content	58.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	L
Subcluster	L2
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Acquire49 (L1) Appletree2 (L1) AvadaKedavra (L1) Calm (L1) CicholasNage (L1) DirkDirk (L1) Enceladus (L1) Halena (L1) JoeDirt (L1) LeBron (L1) MAckerman (L1) OhShagHennessy (L1) Rose5 (L1) Silverleaf (L1) Tyson (L1) UPIE (L1) Wamburgrxpress (L1) Wyatt2 (L1) Zaria (L1) Archie (L2) Baoshan (L2) Bazzle (L2) BigCheese (L2) BobsGarage (L2) Breezona (L2) Claus (L2) Crossroads (L2) DanBing (L2) DHan (L2) DrSeegs (L2) Faith1 (L2) Finemlucis (L2) Gabriela (L2) Gardann (L2) GuuelaD (L2) Hafay (L2) Itos (L2) Kahlid (L2) Lewan (L2) LilDestine (L2) Loadrie (L2) Miley16 (L2) MkaliMitinis3 (L2) Netyap (L2) Nicholasp3 (L2) Rumpelstiltskin (L2) Sarshaun (L2) Soap141 (L2) Tourach (L2) Vetrix (L2) Wigglewiggle (L2) Wilder (L2) Winky (L2) Zakai (L2) ZhongYanYuan (L2) Bellis (L3) BourbonZero (L3) Clautastrophe (L3) DuncansLeg (L3) Ellson (L3) Finnry (L3) Jobypre (L3) Jubie (L3) Kingsolomon (L3) KirDoubleO7 (L3) Krypton555 (L3) LiyuLake (L3) Lolly9 (L3) Lumos (L3) MiniLon (L3) MiniMac (L3) Moostard (L3) MsGreen (L3) Nicholas (L3) Samty (L3) Snenia (L3) Whirlwind (L3) BrainDrainer (L4) Bromden (L4) Chaser (L4) Douge (L4) Douzhi (L4) DyoEdafos (L4) FarmResident (L4) PYPDinur (L4) Quby (L4) Sheng711 (L4) Aegeus (L5) Baudelaire (L5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	132
Number of tRNAs	12
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Breezona_1 Breezona_2 (terminase small subunit) Breezona_3 Breezona_4 (terminase large subunit) Breezona_5 (portal protein) Breezona_6 (MuF-like minor capsid protein) Breezona_7 (scaffolding protein) Breezona_8 (major capsid protein) Breezona_9 (head-to-tail adaptor) Breezona_10 (head-to-tail stopper) Breezona_11 Breezona_12 (tail terminator) Breezona_13 (major tail protein) Breezona_14 Breezona_15 (tail assembly chaperone) Breezona_16 (tape measure protein) Breezona_17 (minor tail protein) Breezona_18 (minor tail protein) Breezona_19 (minor tail protein) Breezona_20 (minor tail protein) Breezona_21 Breezona_22 Breezona_23 Breezona_24 (minor tail protein) Breezona_25 Breezona_26 Breezona_27 (lysin A) Breezona_28 (lysin B) Breezona_29 Breezona_30 Breezona_31 Breezona_32 (NrdH-like glutaredoxin) Breezona_33 Breezona_34 Breezona_35 (nucleotidyl transferase) Breezona_36 (Lsr2-like DNA bridging protein) Breezona_37 Breezona_38 (tyrosine integrase) Breezona_39 Breezona_40 (immunity repressor) Breezona_41 Breezona_42 Breezona_43 Breezona_44 Breezona_45 Breezona_46 Breezona_47 (esterase) Breezona_48 Breezona_49 Breezona_50 Breezona_51 Breezona_52 Breezona_53 Breezona_54 Breezona_55 (Ro-like RNA binding protein) Breezona_56 Breezona_57 Breezona_58 Breezona_59 Breezona_60 Breezona_61 Breezona_62 Breezona_63 (ssDNA binding protein, ERF family) Breezona_64 Breezona_65 Breezona_66 Breezona_67 (HNH endonuclease) Breezona_68 Breezona_69 Breezona_70 (DnaB-like dsDNA helicase) Breezona_71 (WhiB family transcription factor) Breezona_72 Breezona_73 Breezona_74 (phosphoribosyl pyrophosphate transferase) Breezona_75 (nicotinate ribosyltransferase) Breezona_76 (polynucleotide kinase) Breezona_77 Breezona_78 Breezona_79 Breezona_80 (RusA-like resolvase) Breezona_81 (WhiB family transcription factor) Breezona_82 (WhiB family transcription factor) Breezona_83 Breezona_84 Breezona_85 (ssDNA binding protein) Breezona_86 Breezona_87 Breezona_88 (RNA ligase) Breezona_89 Breezona_90 (ClpP-like protease) Breezona_91 Breezona_92 Breezona_93 Breezona_94 Breezona_95 (HNH endonuclease) Breezona_96 Breezona_97 Breezona_98 (DnaQ-like DNA polymerase III subunit) Breezona_99 Breezona_100 Breezona_101 Breezona_102 Breezona_103 Breezona_104 Breezona_105 — tRNA Breezona_106 Breezona_107 — tRNA Breezona_108 — tRNA Breezona_109 — tRNA Breezona_110 Breezona_111 Breezona_112 — tRNA (tRNA Leu (tag)) Breezona_113 — tRNA (tRNA His (gtg)) Breezona_114 — tRNA (tRNA Gln (ctg)) Breezona_115 — tRNA (tRNA Gly (tcc)) Breezona_116 — tRNA (tRNA Lys (ctt)) Breezona_117 Breezona_118 Breezona_119 — tRNA (tRNA Cys (gca)) Breezona_120 Breezona_121 Breezona_122 — tRNA (tRNA Asn (gtt)) Breezona_123 Breezona_124 — tRNA (tRNA Lys (ttt)) Breezona_125 Breezona_126 Breezona_127 Breezona_128 Breezona_129 Breezona_130 Breezona_131 Breezona_132 Breezona_133 Breezona_134 Breezona_135 Breezona_136 Breezona_137 Breezona_138 Breezona_139 Breezona_140 Breezona_141 Breezona_142 Breezona_143 Breezona_144
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KC691254
Refseq Number	NC_021296
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	Buck
Pitt Freezer Box#	21
Pitt Freezer Box Grid#	61
Available Files
EM Picture	Download

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