| Detailed Information for Phage Contagion |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Paola Lockwood |
| Year Found | 2011 |
| Location Found | Westchester, CA USA |
| Finding Institution | Loyola Marymount University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.969875 N, 118.420014 W Map |
| Discovery Notes | Sunny day approximately 69°F. Located on the campus of LMU by the sophomore housing, specifically the covered garden in front of McCarthy Hall. Soil sample was taken 2-3inches below the surface by a blossoming tree's roots. Other plants (flowers,grass) were in the near area. The dirt was moist. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2012 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 80 |
| Genome length (bp) | 74533 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Lysogenic. Fuzzy concentric rings around a cock-eye lysed center. Approximately 0.1 cm in diameter after 24-48 hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 140 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF024732 |
| Refseq Number | NC_022065 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011LOMAcontagionPL |
| SEA Lysate Titer |
2.11e12 |
| Date of SEA Lysate Titering |
Oct 24, 2011 |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
