The Actinobacteriophage Database

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Detailed Information for Phage Dusk
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Abbey Perl
Year Found	2010
Location Found	NJ USA
Finding Institution	Lehigh University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	40.5578 N, 74.8758 W Map
Discovery Notes	This phage was discovered at the bottom of a oak tree. It was taken from a rotting limb from the oak tree. The soil temperature was slightly warm. The soil was moist.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Feb 28, 2011
Genome length (bp)	75339
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka Filch FireRed Flypotenuse Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	The plaque forms small, 2.5 mm diameter, bullseye plaques. They have a very low infectivity and is very cloudy. It looks as if there is a layer of bacteria ontop of the plaque.
Morphotype	Siphoviridae
Number of Genes	142
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Dusk_1 Dusk_2 Dusk_3 Dusk_4 Dusk_5 Dusk_6 (terminase small subunit) Dusk_7 Dusk_8 (terminase large subunit) Dusk_9 Dusk_10 (portal protein) Dusk_11 (capsid maturation protease) Dusk_12 (major capsid protein) Dusk_13 Dusk_14 (head-to-tail adaptor) Dusk_15 (head-to-tail stopper) Dusk_16 (head-to-tail connector) Dusk_17 (tail terminator) Dusk_18 (major tail subunit) Dusk_19 (tail assembly chaperone) Dusk_20 (tail assembly chaperone) Dusk_21 (tape measure protein) Dusk_22 (minor tail protein) Dusk_23 (minor tail protein) Dusk_24 (minor tail protein) Dusk_25 (minor tail protein) Dusk_26 (D-Ala-D-Ala carboxypeptidase) Dusk_27 Dusk_28 Dusk_29 (HTH DNA binding protein) Dusk_30 Dusk_31 (lysin A) Dusk_32 (holin) Dusk_33 Dusk_34 (lysin B) Dusk_35 Dusk_36 (NrdH-like redoxin) Dusk_37 (HNH endonuclease) Dusk_38 Dusk_39 Dusk_40 Dusk_41 Dusk_42 Dusk_43 Dusk_44 Dusk_45 Dusk_46 Dusk_47 Dusk_48 Dusk_49 Dusk_50 (tyrosine integrase) Dusk_51 Dusk_52 Dusk_53 (DNA binding domain protein) Dusk_54 Dusk_55 (pentapeptide repeat protein) Dusk_56 Dusk_57 Dusk_58 Dusk_59 Dusk_60 Dusk_61 Dusk_62 Dusk_63 Dusk_64 Dusk_65 Dusk_66 (ssDNA binding protein, ERF family) Dusk_67 Dusk_68 Dusk_69 Dusk_70 Dusk_71 Dusk_72 Dusk_73 Dusk_74 (HNH endonuclease) Dusk_75 Dusk_76 Dusk_77 Dusk_78 (DnaB-like helicase) Dusk_79 Dusk_80 (WhiB family transcription factor) Dusk_81 Dusk_82 Dusk_83 (holliday junction resolvase) Dusk_84 Dusk_85 (polynucleotide kinase) Dusk_86 Dusk_87 Dusk_88 (RNA ligase) Dusk_89 (HTH DNA binding protein) Dusk_90 (WhiB) Dusk_91 Dusk_92 Dusk_93 Dusk_94 Dusk_95 Dusk_96 (DNA methylase) Dusk_97 (ssDNA binding protein) Dusk_98 (ClpP-like protease) Dusk_99 Dusk_100 Dusk_101 Dusk_102 (HTH DNA binding protein) Dusk_103 (HNH homing endonuclease) Dusk_104 — tRNA (tRNA-Arg (cct)) Dusk_105 — tRNA (tRNA-Gly(tcc)) Dusk_106 Dusk_107 Dusk_108 Dusk_109 Dusk_110 Dusk_111 (DnaQ-like DNA polymerase III subunit) Dusk_112 Dusk_113 Dusk_114 Dusk_115 Dusk_116 (RecA-like DNA recombinase) Dusk_117 Dusk_118 Dusk_119 Dusk_120 Dusk_121 Dusk_122 Dusk_123 Dusk_124 Dusk_125 Dusk_126 Dusk_127 Dusk_128 (HTH DNA binding protein) Dusk_129 Dusk_130 Dusk_131 Dusk_132 Dusk_133 Dusk_134 Dusk_135 Dusk_136 Dusk_137 Dusk_138 Dusk_139 Dusk_140 Dusk_141 Dusk_142 Dusk_143 Dusk_144
Submitted Annotation in DNA Master Format	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KT222942
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	15
Pitt Freezer Box Grid#	14

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