Mycobacterium phage Gardann
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Detailed Information for Phage Gardann
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGeorgeanna Holmes
Year Found2011
Location FoundSuwanee, GA USA
Finding InstitutionGeorgia Gwinnett College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates34.083392 N, 84.067072 W Map
Discovery NotesCompost pile in a garden. 1 inch from surface. dug around 8:30 am. around 80 degrees Fahrenheit. dug on September 20, 2011. elevation 1018 ft.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 4, 2015
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage40
Sanger Finishing Reactions8
Genome length (bp)76012
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notesdiameter: .5mm. hazy edges, clear center. pretty much stayed right at .5 mm the whole time except one generation it was .6 mm.
MorphotypeSiphoviridae
Number of Genes126
Number of tRNAs14
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Gardann_1

Gardann_2
(terminase small subunit)

Gardann_3

Gardann_4
(terminase large subunit)

Gardann_5
(portal protein)

Gardann_6
(MuF-like minor capsid protein)

Gardann_7
(scaffolding protein)

Gardann_8
(major capsid protein)

Gardann_9
(head-to-tail adaptor)

Gardann_10
(head-to-tail stopper)

Gardann_11

Gardann_12
(tail terminator)

Gardann_13
(major tail protein)

Gardann_14
(tail assembly chaperone)

Gardann_15
(tail assembly chaperone)

Gardann_16
(tape measure protein)

Gardann_17
(minor tail protein)

Gardann_18
(minor tail protein)

Gardann_19
(minor tail protein)

Gardann_20
(minor tail protein)

Gardann_21
(minor tail protein)

Gardann_22
(minor tail protein)

Gardann_23

Gardann_24
(minor tail protein)

Gardann_25

Gardann_26

Gardann_27
(lysin A)

Gardann_28
(lysin B)

Gardann_29

Gardann_30

Gardann_31

Gardann_32
(NrdH-like glutaredoxin)

Gardann_33

Gardann_34

Gardann_35
(nucleotidyl transferase)

Gardann_36
(Lsr2-like DNA bridging protein)

Gardann_37

Gardann_38
(tyrosine integrase)

Gardann_39

Gardann_40
(immunity repressor)

Gardann_41
(Cro protein)

Gardann_42
(excise)

Gardann_43

Gardann_44

Gardann_45

Gardann_46

Gardann_47
(esterase)

Gardann_48

Gardann_49

Gardann_50

Gardann_51

Gardann_52

Gardann_53

Gardann_54

Gardann_55
(Ro-like RNA binding protein)

Gardann_56 — tRNA

Gardann_57

Gardann_58

Gardann_59

Gardann_60

Gardann_61

Gardann_62

Gardann_63

Gardann_64
(ssDNA binding protein, ERF family)

Gardann_65

Gardann_66

Gardann_67

Gardann_68
(HNH endonuclease)

Gardann_69

Gardann_70

Gardann_71
(DnaB-like dsDNA helicase)

Gardann_72
(WhiB family transcription factor)

Gardann_73

Gardann_74

Gardann_75
(phosphoribosyl pyrophosphate transferase)

Gardann_76
(nicotinate ribosyltransferase)

Gardann_77
(polynucleotide kinase)

Gardann_78

Gardann_79

Gardann_80
(RusA-like resolvase)

Gardann_81
(WhiB family transcription factor)

Gardann_82
(WhiB family transcription factor)

Gardann_83

Gardann_84

Gardann_85
(ssDNA binding protein)

Gardann_86

Gardann_87

Gardann_88
(RNA ligase)

Gardann_89

Gardann_90
(ClpP-like protease)

Gardann_91

Gardann_92

Gardann_93

Gardann_94

Gardann_95
(HNH endonuclease)

Gardann_96

Gardann_97

Gardann_98
(DnaQ-like DNA polymerase III subunit)

Gardann_99

Gardann_100

Gardann_101

Gardann_102

Gardann_103

Gardann_104

Gardann_105 — tRNA

Gardann_106

Gardann_107 — tRNA

Gardann_108 — tRNA

Gardann_109 — tRNA

Gardann_110 — tRNA

Gardann_111 — tRNA

Gardann_112 — tRNA

Gardann_113 — tRNA

Gardann_114 — tRNA

Gardann_115 — tRNA
(tRNA-Ser(uga))

Gardann_116

Gardann_117

Gardann_118 — tRNA

Gardann_119

Gardann_120

Gardann_121 — tRNA

Gardann_122

Gardann_123 — tRNA

Gardann_124

Gardann_125

Gardann_126

Gardann_127

Gardann_128

Gardann_129

Gardann_130

Gardann_131

Gardann_132

Gardann_133

Gardann_134

Gardann_135

Gardann_136

Gardann_137

Gardann_138
(AAA-ATPase)

Gardann_139

Gardann_140

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX507361
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2011GAGWgardannGH
Pitt Freezer Box# 23
Pitt Freezer Box Grid# 15
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload