| Detailed Information for Phage Goldilocks |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Evelyn Lewis and Grahm Skowronski |
| Year Found | 2015 |
| Location Found | Norfolk, VA USA |
| Finding Institution | Old Dominion University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.885900 N, 76.305700 W Map |
| Naming Notes | It took a while to get the conditions just right for this phage to be properly purified thus affectionately being named Goldilocks |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 366 |
| Genome length (bp) | 75728 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Goldilocks displays multiple plaque morphological types; a 0.5mm temperate plaque and a 2.5mm temperate plaque. A total of 10 purifications were done before doing an EM that showed homogeneous viruses. |
| Morphotype | Siphoviridae |
| Number of Genes | 150 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX834009 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
22 |
| Pitt Freezer Box Grid# |
E1 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |