Mycobacterium phage Jobypre
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Detailed Information for Phage Jobypre
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByChrista Joby
Year Found2022
Location FoundCharleston, SC United States
Finding InstitutionCollege of Charleston
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.786463 N, 79.938603 W Map
Discovery NotesSoil sample taken from the base of a palmetto tree on the side of the road in Charleston, SC. Weather was 85˚F with slight humidity. Soil was moist because it had been raining. Nearby vegetation included a few bushes and trees.
Naming NotesThis phage is named after my father. His first name is Joby. I added "pre" so that the name would be accepted since there was already a phage named Joby.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 20, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage57
Genome length (bp)75624
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionCollege of Charleston
Annotation StatusQC Complete
MorphotypeSiphoviridae
Number of Genes128
Number of tRNAs10
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Jobypre_1

Jobypre_2
(terminase, small subunit)

Jobypre_3

Jobypre_4

Jobypre_5
(terminase, large subunit)

Jobypre_6
(portal protein)

Jobypre_7
(minor capsid protein)

Jobypre_8
(scaffolding protein)

Jobypre_9
(major capsid protein)

Jobypre_10
(head-to-tail adaptor)

Jobypre_11
(head-to-tail stopper)

Jobypre_12

Jobypre_13
(tail terminator)

Jobypre_14
(major tail protein)

Jobypre_15
(tail assembly chaperone)

Jobypre_16
(tail assembly chaperone)

Jobypre_17
(tape measure protein)

Jobypre_18
(minor tail protein)

Jobypre_19
(minor tail protein)

Jobypre_20

Jobypre_21
(minor tail protein)

Jobypre_22
(minor tail protein)

Jobypre_23

Jobypre_24

Jobypre_25

Jobypre_26
(lysin A)

Jobypre_27
(holin)

Jobypre_28
(lysin B)

Jobypre_29

Jobypre_30

Jobypre_31
(NrdH-like glutaredoxin)

Jobypre_32

Jobypre_33
(nucleotidyl transferase)

Jobypre_34
(Lsr2-like DNA bridging protein)

Jobypre_35

Jobypre_36
(tyrosine integrase)

Jobypre_37

Jobypre_38
(immunity repressor)

Jobypre_39

Jobypre_40
(excise)

Jobypre_41

Jobypre_42

Jobypre_43

Jobypre_44

Jobypre_45
(esterase)

Jobypre_46

Jobypre_47

Jobypre_48

Jobypre_49
(membrane protein, Band-7 -like)

Jobypre_50 — tRNA
(tRNA-Tyr(gta))

Jobypre_51

Jobypre_52

Jobypre_53

Jobypre_54

Jobypre_55
(Ro-like RNA binding protein)

Jobypre_56

Jobypre_57

Jobypre_58

Jobypre_59

Jobypre_60

Jobypre_61

Jobypre_62
(ERF family DNA pairing protein)

Jobypre_63

Jobypre_64
(ParB-like nuclease domain)

Jobypre_65

Jobypre_66
(HNH endonuclease)

Jobypre_67

Jobypre_68

Jobypre_69
(DnaB-like dsDNA helicase)

Jobypre_70
(WhiB family transcription factor)

Jobypre_71

Jobypre_72

Jobypre_73

Jobypre_74
(phosphoribosyltransferase)

Jobypre_75
(nicotinate ribosyltransferase)

Jobypre_76
(polynucleotide kinase)

Jobypre_77

Jobypre_78
(RusA-like resolvase)

Jobypre_79
(WhiB family transcription factor)

Jobypre_80
(WhiB family transcription factor)

Jobypre_81

Jobypre_82

Jobypre_83

Jobypre_84
(SSB protein)

Jobypre_85
(toxin in toxin/antitoxin system, HicA-like)

Jobypre_86
(RNA ligase)

Jobypre_87
(ClpP-like protease)

Jobypre_88

Jobypre_89

Jobypre_90

Jobypre_91

Jobypre_92
(HNH endonuclease)

Jobypre_93

Jobypre_94

Jobypre_95
(DnaQ-like (DNA polymerase III subunit))

Jobypre_96

Jobypre_97

Jobypre_98
(HNH endonuclease)

Jobypre_99

Jobypre_100

Jobypre_101

Jobypre_102

Jobypre_103 — tRNA
(tRNA-Thr(cgt))

Jobypre_104

Jobypre_105 — tRNA
(tRNA-Pro(tgg))

Jobypre_106

Jobypre_107 — tRNA
(tRNA-Trp(cca))

Jobypre_108 — tRNA
(tRNA-Leu(tag))

Jobypre_109 — tRNA
(tRNA-His(gtg))

Jobypre_110

Jobypre_111 — tRNA
(tRNA-Gln(ctg))

Jobypre_112 — tRNA
(tRNA-Lys(ctt))

Jobypre_113

Jobypre_114

Jobypre_115 — tRNA
(tRNA-Pyl(cta))

Jobypre_116 — tRNA
(tRNA-Lys(ttt))

Jobypre_117

Jobypre_118

Jobypre_119

Jobypre_120

Jobypre_121

Jobypre_122

Jobypre_123

Jobypre_124

Jobypre_125

Jobypre_126

Jobypre_127

Jobypre_128

Jobypre_129

Jobypre_130

Jobypre_131

Jobypre_132

Jobypre_133

Jobypre_134
(AAA-ATPase)

Jobypre_135

Jobypre_136

Jobypre_137

Jobypre_138

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 163
Pitt Freezer Box Grid# H8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload