| Detailed Information for Phage Jobypre |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Christa Joby |
| Year Found | 2022 |
| Location Found | Charleston, SC United States |
| Finding Institution | College of Charleston |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.786463 N, 79.938603 W Map |
| Discovery Notes | Soil sample taken from the base of a palmetto tree on the side of the road in Charleston, SC. Weather was 85˚F with slight humidity. Soil was moist because it had been raining. Nearby vegetation included a few bushes and trees. |
| Naming Notes | This phage is named after my father. His first name is Joby. I added "pre" so that the name would be accepted since there was already a phage named Joby. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 57 |
| Genome length (bp) | 75624 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | College of Charleston |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 128 |
| Number of tRNAs | 10 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV251868 |
| Refseq Number | None yet |
| Published in Paper | Polic et al 2025, Microbiology Resource Announcements: Complete genome sequence of Jobypre, an L3 subcluster mycobacteriophage isolated from marsh soil in Charleston, South Carolina |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
163 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
