| Detailed Information for Phage Kanye |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alexander Adia |
| Year Found | 2014 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.824255 N, 71.403221 W Map |
| Discovery Notes | The soil sample was taken from a tree in front of Everett House on Keeney Quad. The soil was particularly moist because it had rained on Providence shortly before I collected my sample and because I collected my soil less than a foot from the base of the tree. |
| Naming Notes | My first thought when I saw my phage was that it had a big head. I also thought it would be a privilege to say that Kanye had, quite literally, gone viral.
While I was initially devastated that I couldn't come up with a pun involving phage due to the naming rules (Ellen Phage and Nacho Phage were casualties of such rules), Kanye was a very welcome consolation prize. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 28, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1621 |
| Genome length (bp) | 75453 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were always clear and and about 1-2 mm in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 140 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN428059 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.6x10^10 pfus/mL |
| Date of SEA Lysate Titering |
Dec 3, 2014 |
| Pitt Freezer Box# |
11 |
| Pitt Freezer Box Grid# |
F5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
