| Detailed Information for Phage Krypton555 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kelsey Green |
| Year Found | 2016 |
| Location Found | New Orleans, LA USA |
| Finding Institution | Xavier University of Louisiana |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.97 N, 90.11 W Map |
| Naming Notes | I named my phage Krypton-555 because I believe that it's strength throughout the whole discovery process was excellent. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1875 |
| Genome length (bp) | 76075 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.3% |
| Sequencing Notes | at position 21051 bp 20% of reads contain an A instead of consensus C
at position 21188 bp 30% of reads contain an A instead of consensus G |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Small, almost perfectly round spots |
| Morphotype | Siphoviridae |
| Number of Genes | 130 |
| Number of tRNAs | 11 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF140414 |
| Refseq Number | None yet |
| Published in Paper | Chung HM et al 2017, Genome Announcements: Complete Genome Sequences of Mycobacteriophages Clautastrophe, Kingsolomon, Krypton555, and Nicholas |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3e10 |
| Date of SEA Lysate Titering |
Nov 5, 2016 |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
