Mycobacterium phage Krypton555
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Detailed Information for Phage Krypton555
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKelsey Green
Year Found2016
Location FoundNew Orleans, LA USA
Finding InstitutionXavier University of Louisiana
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates29.97 N, 90.11 W Map
Naming NotesI named my phage Krypton-555 because I believe that it's strength throughout the whole discovery process was excellent.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1875
Genome length (bp)76075
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.3%
Sequencing Notesat position 21051 bp 20% of reads contain an A instead of consensus C
at position 21188 bp 30% of reads contain an A instead of consensus G
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesSmall, almost perfectly round spots
MorphotypeSiphoviridae
Number of Genes130
Number of tRNAs11
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Krypton555_1

Krypton555_2

Krypton555_3

Krypton555_4

Krypton555_5
(terminase large subunit)

Krypton555_6
(portal protein)

Krypton555_7
(MuF-like minor capsid protein)

Krypton555_8
(scaffolding protein)

Krypton555_9
(major capsid protein)

Krypton555_10
(head-to-tail adaptor)

Krypton555_11
(head-to-tail stopper)

Krypton555_12

Krypton555_13
(tail terminator)

Krypton555_14
(major tail protein)

Krypton555_16
(tail assembly chaperone)

Krypton555_15
(tail assembly chaperone)

Krypton555_17
(tape measure protein)

Krypton555_18
(minor tail protein)

Krypton555_19
(minor tail protein)

Krypton555_20
(minor tail protein)

Krypton555_21
(minor tail protein)

Krypton555_22
(minor tail protein)

Krypton555_23

Krypton555_24

Krypton555_25

Krypton555_26
(lysin A)

Krypton555_27
(holin)

Krypton555_28
(lysin B)

Krypton555_29

Krypton555_30

Krypton555_31
(NrdH-like glutaredoxin)

Krypton555_32

Krypton555_33
(nucleotidyltransferase)

Krypton555_34
(Lsr2-like DNA bridging protein)

Krypton555_35

Krypton555_36
(tyrosine integrase)

Krypton555_37

Krypton555_38

Krypton555_39
(Cro protein)

Krypton555_40
(excise)

Krypton555_41

Krypton555_42

Krypton555_43

Krypton555_44

Krypton555_45

Krypton555_46
(esterase)

Krypton555_47

Krypton555_48

Krypton555_49

Krypton555_50

Krypton555_51 — tRNA

Krypton555_52

Krypton555_53

Krypton555_54

Krypton555_55

Krypton555_56
(Ro-like RNA binding protein)

Krypton555_57

Krypton555_58

Krypton555_59

Krypton555_60

Krypton555_61

Krypton555_62

Krypton555_63
(ERF family ssDNA binding protein)

Krypton555_64

Krypton555_65

Krypton555_66

Krypton555_67
(HNH endonuclease)

Krypton555_68

Krypton555_69

Krypton555_70
(DnaB-like dsDNA helicase)

Krypton555_71
(WhiB family transcription factor)

Krypton555_72

Krypton555_73

Krypton555_74

Krypton555_75
(phosphoribosyl pyrophosphate transferase)

Krypton555_76
(nicotinate ribosyltransferase)

Krypton555_77
(polynucleotide kinase)

Krypton555_78

Krypton555_79
(RusA-like resolvase)

Krypton555_80
(WhiB family transcription factor)

Krypton555_81
(WhiB family transcription factor)

Krypton555_82

Krypton555_83

Krypton555_84
(ssDNA-binding protein)

Krypton555_85

Krypton555_86

Krypton555_87
(RNA ligase)

Krypton555_88
(ClpP-like protease)

Krypton555_89

Krypton555_90

Krypton555_91

Krypton555_92

Krypton555_93

Krypton555_94
(HNH endonuclease)

Krypton555_95

Krypton555_96

Krypton555_97
(DnaQ-like DNA polymerase III subunit)

Krypton555_98

Krypton555_99

Krypton555_100

Krypton555_101
(HNH endonuclease)

Krypton555_102

Krypton555_103

Krypton555_104

Krypton555_105

Krypton555_106 — tRNA

Krypton555_107

Krypton555_108 — tRNA

Krypton555_109

Krypton555_110 — tRNA

Krypton555_111 — tRNA

Krypton555_112 — tRNA

Krypton555_113 — tRNA

Krypton555_114

Krypton555_115 — tRNA

Krypton555_116 — tRNA

Krypton555_117

Krypton555_118

Krypton555_119 — tRNA

Krypton555_120 — tRNA

Krypton555_121

Krypton555_122

Krypton555_123

Krypton555_124

Krypton555_125

Krypton555_126

Krypton555_127

Krypton555_128

Krypton555_129

Krypton555_130

Krypton555_131

Krypton555_132

Krypton555_133

Krypton555_134

Krypton555_135

Krypton555_136
(AAA-ATPase)

Krypton555_137

Krypton555_138

Krypton555_139

Krypton555_140

Krypton555_141

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140414
Refseq NumberNone yet
Published in PaperChung HM et al 2017, Genome Announcements: Complete Genome Sequences of Mycobacteriophages Clautastrophe, Kingsolomon, Krypton555, and Nicholas
Archiving Info
Archiving status Archived
SEA Lysate Titer 3e10
Date of SEA Lysate Titering Nov 5, 2016
Pitt Freezer Box# 45
Pitt Freezer Box Grid# H3
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload