Mycobacterium phage Loadrie
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Detailed Information for Phage Loadrie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAdrie Shen and Lauren Sanders
Year Found2013
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates33.212167 N, 97.148217 W Map
Discovery NotesFlower bed outside of Sycamore Hall. The soil was moist and clumpy. It was found around heavy foot traffic.
Naming NotesWe are not very creative, so we combined our names.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2014
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage426
Genome length (bp)76492
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques are approximately 1.5 mm in diameter, with a bulls-eyed ring. Inner circle was completely clear surrounded by a cloudy clearing.
MorphotypeSiphoviridae
Number of Genes133
Number of tRNAs11
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Loadrie_1

Loadrie_2
(terminase small subunit)

Loadrie_3

Loadrie_4
(terminase large subunit)

Loadrie_5
(portal protein)

Loadrie_6
(MuF-like minor capsid protein)

Loadrie_7
(scaffolding protein)

Loadrie_8
(major capsid protein)

Loadrie_9
(head-to-tail adaptor)

Loadrie_10
(head-to-tail stopper)

Loadrie_11

Loadrie_12
(tail terminator)

Loadrie_13
(major tail protein)

Loadrie_14
(tail assembly chaperone)

Loadrie_15
(tail assembly chaperone)

Loadrie_16
(tape measure protein)

Loadrie_17
(minor tail protein)

Loadrie_18
(minor tail protein)

Loadrie_19
(minor tail protein)

Loadrie_20
(minor tail protein)

Loadrie_21

Loadrie_22

Loadrie_23

Loadrie_24
(minor tail protein)

Loadrie_25

Loadrie_26

Loadrie_27
(lysin A)

Loadrie_28
(lysin B)

Loadrie_29

Loadrie_30

Loadrie_31

Loadrie_32
(NrdH-like glutaredoxin)

Loadrie_33

Loadrie_34

Loadrie_35
(nucleotidyl transferase)

Loadrie_36
(Lsr2-like DNA bridging protein)

Loadrie_37

Loadrie_38
(tyrosine integrase)

Loadrie_39

Loadrie_40
(immunity repressor)

Loadrie_41
(Cro protein)

Loadrie_42
(excise)

Loadrie_43

Loadrie_44

Loadrie_45

Loadrie_46
(esterase)

Loadrie_47

Loadrie_48

Loadrie_49

Loadrie_50

Loadrie_51

Loadrie_52

Loadrie_53

Loadrie_54
(Ro-like RNA binding protein)

Loadrie_55

Loadrie_56

Loadrie_57

Loadrie_58

Loadrie_59

Loadrie_60

Loadrie_61

Loadrie_62
(ssDNA binding protein, ERF family)

Loadrie_63

Loadrie_64

Loadrie_65

Loadrie_66
(HNH endonuclease)

Loadrie_67

Loadrie_68

Loadrie_69
(DnaB-like dsDNA helicase)

Loadrie_70
(WhiB family transcription factor)

Loadrie_71

Loadrie_72

Loadrie_73
(phosphoribosyl pyrophosphate transferase)

Loadrie_74
(nicotinate ribosyltransferase)

Loadrie_75
(polynucleotide kinase)

Loadrie_76

Loadrie_77

Loadrie_78

Loadrie_79
(RusA-like resolvase)

Loadrie_80
(WhiB family transcription factor)

Loadrie_81
(WhiB family transcription factor)

Loadrie_82

Loadrie_83
(ssDNA binding protein)

Loadrie_84

Loadrie_85

Loadrie_86

Loadrie_87
(RNA ligase)

Loadrie_88

Loadrie_89
(ClpP-like protease)

Loadrie_90

Loadrie_91

Loadrie_92

Loadrie_93

Loadrie_94
(HNH endonuclease)

Loadrie_95

Loadrie_96

Loadrie_97
(DnaQ-like DNA polymerase III subunit)

Loadrie_98

Loadrie_99

Loadrie_100

Loadrie_101

Loadrie_102

Loadrie_103

Loadrie_104

Loadrie_105 — tRNA
(tRNA-Thr(cgt))

Loadrie_106

Loadrie_107

Loadrie_108 — tRNA
(tRNA-Pro(tgg))

Loadrie_109 — tRNA
(tRNA-Trp(cca))

Loadrie_110

Loadrie_111

Loadrie_112 — tRNA
(tRNA-Leu(tag))

Loadrie_113 — tRNA
(tRNA-His(gtg))

Loadrie_114 — tRNA
(tRNA-Gln(ctg))

Loadrie_115 — tRNA
(tRNA-Gly(tcc))

Loadrie_116 — tRNA
(tRNA-Lys(ctt))

Loadrie_117

Loadrie_118

Loadrie_119 — tRNA
(tRNA-Cys(gca))

Loadrie_120

Loadrie_121

Loadrie_122

Loadrie_123 — tRNA
(tRNA-Asn(gtt))

Loadrie_124

Loadrie_125 — tRNA
(tRNA-Lys(ttt))

Loadrie_126

Loadrie_127

Loadrie_128

Loadrie_129

Loadrie_130

Loadrie_131

Loadrie_132

Loadrie_133

Loadrie_134

Loadrie_135

Loadrie_136

Loadrie_137

Loadrie_138

Loadrie_139
(AAA-ATPase)

Loadrie_140

Loadrie_141

Loadrie_142

Loadrie_143

Loadrie_144

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU997639
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 2
Pitt Freezer Box Grid# B12