Mycobacterium phage Miley16
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Detailed Information for Phage Miley16
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesReginaGeorge
Found ByMia Comis
Year Found2016
Location FoundFarmington, ME USA
Finding InstitutionUniversity of Maine, Farmington
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates44.6652905 N, 70.1466901 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage463
Genome length (bp)76653
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPinpoint-sized circular plaques.
MorphotypeSiphoviridae
Number of Genes132
Number of tRNAs12
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Miley16_1

Miley16_2
(terminase small subunit)

Miley16_3

Miley16_4
(terminase large subunit)

Miley16_5
(portal protein)

Miley16_6
(MuF-like minor capsid protein)

Miley16_7
(scaffolding protein)

Miley16_8
(major capsid protein)

Miley16_9
(head-to-tail adaptor)

Miley16_10
(head-to-tail stopper)

Miley16_11

Miley16_12
(tail terminator)

Miley16_13
(major tail protein)

Miley16_14
(tail assembly chaperone)

Miley16_15
(tail assembly chaperone)

Miley16_16
(tape measure protein)

Miley16_17
(minor tail protein)

Miley16_18
(minor tail protein)

Miley16_19
(minor tail protein)

Miley16_20
(minor tail protein)

Miley16_21
(minor tail protein)

Miley16_22
(minor tail protein)

Miley16_23

Miley16_24
(minor tail protein)

Miley16_25

Miley16_26

Miley16_27
(lysin A)

Miley16_28
(lysin B)

Miley16_29

Miley16_30

Miley16_31

Miley16_32
(NrdH-like glutaredoxin)

Miley16_33

Miley16_34

Miley16_35
(nucleotidyltransferase)

Miley16_36
(Lsr2-like DNA bridging protein)

Miley16_37

Miley16_38
(tyrosine integrase)

Miley16_39

Miley16_40
(immunity repressor)

Miley16_41
(Cro protein)

Miley16_42
(excise)

Miley16_43

Miley16_44

Miley16_45

Miley16_46

Miley16_47
(esterase)

Miley16_48

Miley16_49

Miley16_50

Miley16_51

Miley16_52

Miley16_53

Miley16_54

Miley16_55
(Ro-like RNA binding protein)

Miley16_56

Miley16_57

Miley16_58

Miley16_59

Miley16_60

Miley16_61

Miley16_62

Miley16_63
(ERF family ssDNA binding protein)

Miley16_64

Miley16_65

Miley16_66

Miley16_67
(HNH endonuclease)

Miley16_68

Miley16_69

Miley16_70
(DnaB-like dsDNA helicase)

Miley16_71
(WhiB family transcription factor)

Miley16_72

Miley16_73

Miley16_74
(phosphoribosyl pyrophosphate transferase)

Miley16_75
(nicotinate ribosyltransferase)

Miley16_76
(polynucleotide kinase)

Miley16_77

Miley16_78

Miley16_79

Miley16_80
(RusA-like resolvase)

Miley16_81
(WhiB family transcription factor)

Miley16_82
(WhiB family transcription factor)

Miley16_83

Miley16_84

Miley16_85
(ssDNA-binding protein)

Miley16_86

Miley16_87

Miley16_88
(RNA ligase)

Miley16_89

Miley16_90
(ClpP-like protease)

Miley16_91

Miley16_92

Miley16_93

Miley16_94

Miley16_95
(HNH endonuclease)

Miley16_96

Miley16_97

Miley16_98
(DnaQ-like DNA polymerase III subunit)

Miley16_99

Miley16_100

Miley16_101
(HNH endonuclease)

Miley16_102

Miley16_103

Miley16_104

Miley16_105 — tRNA

Miley16_106

Miley16_107 — tRNA

Miley16_108 — tRNA

Miley16_109 — tRNA

Miley16_110

Miley16_111

Miley16_112 — tRNA

Miley16_113 — tRNA

Miley16_114 — tRNA

Miley16_115 — tRNA

Miley16_116 — tRNA

Miley16_117

Miley16_118

Miley16_119 — tRNA

Miley16_120

Miley16_121

Miley16_122 — tRNA

Miley16_123

Miley16_124 — tRNA

Miley16_125

Miley16_126

Miley16_127

Miley16_128

Miley16_129

Miley16_130

Miley16_131

Miley16_132

Miley16_133

Miley16_134

Miley16_135

Miley16_136

Miley16_137

Miley16_138

Miley16_139
(AAA-ATPase)

Miley16_140

Miley16_141

Miley16_142

Miley16_143

Miley16_144

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF185730
Refseq NumberNone yet
Published in PaperHerren et al. 2017, Genome Announcements: Genome Sequences of Four Subcluster L2 Mycobacterium Phages, Finemlucis, Miley16, Wilder, and Zakai
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.22X10^11
Date of SEA Lysate Titering Nov 2, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# H9
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload