| Detailed Information for Phage MiniMac |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Caitlin McCaffrey |
| Year Found | 2018 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 29.81808 S, 30.94255 E Map |
| Discovery Notes | MiniMac was discovered beneath a small tree in a flowerbed found in the car park of the finance and administration department (above the cafeteria) at the University of KwaZulu-Natal. The soil sample appeared light in colour and relatively dry which could be owing to the lack of rain in recent weeks. The phage plaques were visible on the agar plates approximately 24-36 hours after plating, and appeared first following a spot test of the environmental sample's filtrate. |
| Naming Notes | The name of this mycobacteriophage originated from my surname being McCaffrey. My mother - Sarah McCaffrey - is known by her close friends as Mac, this filtered down to my nickname being little Mac. MiniMac, therefore, being the logical name for the newest and smallest addition to the family. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 9, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 714 |
| Genome length (bp) | 70064 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Kwazulu-Natal |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 117 |
| Number of tRNAs | 11 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT889391 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
96 |
| Pitt Freezer Box Grid# |
C10 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
