| Detailed Information for Phage MsGreen |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nonhle Mkhwanazi |
| Year Found | 2015 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.9 S, 30.9 E Map |
| Discovery Notes | The soil was sampled in a backyard where my family hang washed laundry |
| Naming Notes | MsGreen was named after a a weird luminous green snake with black stripes once seen near the spot where we hang dry washed laundry |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 17, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2367 |
| Genome length (bp) | 75617 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Pin point clear plaques during the 1st 2 days of incubation. Halo plaques with 1mm diameter after 3 days |
| Morphotype | Siphoviridae |
| Number of Genes | 128 |
| Number of tRNAs | 10 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK878900 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
A11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
