Mycobacterium phage Myrale
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Detailed Information for Phage Myrale
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesWrex
Found ByRyan Veilleux, Jarid Walton
Year Found2017
Location FoundFort Kent, ME USA
Finding InstitutionUniversity of Maine, Fort Kent
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates47.250312 N, 68.586886 W Map
Discovery NotesThe Sample came from the University Campus Garden and this phage came from the same Soil Sample as Ashen and Mayo.
Naming NotesIt was named after a character in a video game.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1927
Genome length (bp)76419
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes144
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Myrale_1

Myrale_2

Myrale_3

Myrale_4

Myrale_5

Myrale_6
(terminase small subunit)

Myrale_7

Myrale_8

Myrale_9
(terminase large subunit)

Myrale_10

Myrale_11
(portal protein)

Myrale_12
(capsid maturation protease)

Myrale_13
(major capsid protein)

Myrale_14

Myrale_15
(head-to-tail adaptor)

Myrale_16
(head-to-tail stopper)

Myrale_17

Myrale_18
(tail terminator)

Myrale_19
(major tail protein)

Myrale_20
(tail assembly chaperone)

Myrale_21
(tail assembly chaperone)

Myrale_22
(tape measure protein)

Myrale_23
(minor tail protein)

Myrale_24
(minor tail protein)

Myrale_25
(minor tail protein)

Myrale_26
(minor tail protein)

Myrale_27
(minor tail protein)

Myrale_28

Myrale_29

Myrale_30
(helix-turn-helix DNA-binding domain protein)

Myrale_31

Myrale_32
(lysin A)

Myrale_33
(holin)

Myrale_34

Myrale_35
(lysin B)

Myrale_36

Myrale_37
(NrdH-like glutaredoxin)

Myrale_38

Myrale_39

Myrale_40
(Lsr2-like DNA bridging protein)

Myrale_41

Myrale_42

Myrale_43

Myrale_44

Myrale_45

Myrale_46

Myrale_47

Myrale_48

Myrale_49

Myrale_50

Myrale_51

Myrale_52
(tyrosine integrase)

Myrale_53

Myrale_54

Myrale_55
(immunity repressor)

Myrale_56

Myrale_57

Myrale_58
(pentapeptide repeat protein)

Myrale_59

Myrale_60

Myrale_61

Myrale_62

Myrale_63

Myrale_64

Myrale_65

Myrale_66

Myrale_67
(exonuclease)

Myrale_68

Myrale_69
(ERF family ssDNA binding protein)

Myrale_70

Myrale_71

Myrale_72

Myrale_73

Myrale_74

Myrale_75

Myrale_76
(DprA-like DNA processing chain A)

Myrale_77
(HNH endonuclease)

Myrale_78

Myrale_79

Myrale_80

Myrale_81
(DnaB-like DNA helicase)

Myrale_82

Myrale_83
(WhiB family transcription factor)

Myrale_84

Myrale_85

Myrale_86
(holliday junction resolvase)

Myrale_87

Myrale_88
(polynucleotide kinase)

Myrale_89

Myrale_90

Myrale_91
(RNA ligase)

Myrale_92
(helix-turn-helix DNA-binding domain protein)

Myrale_93
(WhiB family transcription factor)

Myrale_94

Myrale_95

Myrale_96

Myrale_97

Myrale_98

Myrale_99
(endonuclease VII)

Myrale_100
(ssDNA binding protein)

Myrale_101
(ClpP-like protease)

Myrale_102

Myrale_103

Myrale_104

Myrale_105
(excise)

Myrale_106
(HNH endonuclease)

Myrale_107 — tRNA
(tRNA-Arg(cct))

Myrale_108 — tRNA
(tRNA-Gly(tcc))

Myrale_109

Myrale_110

Myrale_111
(deoxycytidylate deaminase)

Myrale_112

Myrale_113

Myrale_114
(DnaQ-like DNA polymerase III subunit)

Myrale_115

Myrale_116
(RecA-like DNA recombinase)

Myrale_117

Myrale_118

Myrale_119

Myrale_120

Myrale_121

Myrale_122

Myrale_123

Myrale_124

Myrale_125

Myrale_126

Myrale_127

Myrale_128
(helix-turn-helix DNA-binding domain protein)

Myrale_129

Myrale_130

Myrale_131

Myrale_132

Myrale_133

Myrale_134

Myrale_135

Myrale_136

Myrale_137

Myrale_138

Myrale_139

Myrale_140

Myrale_141

Myrale_142

Myrale_143

Myrale_144

Myrale_145

Myrale_146
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN586051
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.3 x 10^9 pfu/ml
Pitt Freezer Box# 60
Pitt Freezer Box Grid# E8
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload