| Detailed Information for Phage NelitzaMV |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Yanilda Martinez Vega |
| Year Found | 2011 |
| Location Found | Richmond, VA USA |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.625814 N, 77.554336 W Map |
| Discovery Notes | Soil sample was taken from an apartment complex garden, under a tree. Temperature: 74ºF. Depth: 0-5cm, moist soil. Time: 7:15am, 09/21/2011. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 18, 2012 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Genome length (bp) | 72790 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Varied plaque morphology. Smaller plaques (1mm) are clear. Larger plaques (3mm) are clear in the center with a halo. |
| Morphotype | Siphoviridae |
| Number of Genes | 139 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT222941 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011VACUNelitzaMVYMV |
| SEA Lysate Titer |
2.9e9 |
| Date of SEA Lysate Titering |
Nov 3, 2011 |
| Pitt Freezer Box# |
30 |
| Pitt Freezer Box Grid# |
38 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |