Mycobacterium phage Nicholasp3
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Detailed Information for Phage Nicholasp3
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByZach C & Raul O
Year Found2012
Location FoundEl Paso, TX USA
Finding InstitutionUniversity of Texas at El Paso
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates31.8 N, 106.44 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage1060
Sanger Finishing Reactions15
Genome length (bp)75822
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes130
Number of tRNAs12
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Nicholasp3_1

Nicholasp3_2

Nicholasp3_3

Nicholasp3_4
(terminase large subunit)

Nicholasp3_5
(portal protein)

Nicholasp3_6
(MuF-like minor capsid protein)

Nicholasp3_7
(scaffolding protein)

Nicholasp3_8
(major capsid protein)

Nicholasp3_9
(head-to-tail adaptor)

Nicholasp3_10
(head-to-tail stopper)

Nicholasp3_11

Nicholasp3_12
(tail terminator)

Nicholasp3_13
(major tail protein)

Nicholasp3_14
(tail assembly chaperone)

Nicholasp3_15
(tail assembly chaperone)

Nicholasp3_16
(tape measure protein)

Nicholasp3_17
(minor tail protein)

Nicholasp3_18
(minor tail protein)

Nicholasp3_19
(minor tail protein)

Nicholasp3_20
(minor tail protein)

Nicholasp3_21
(minor tail protein)

Nicholasp3_22
(minor tail protein)

Nicholasp3_23

Nicholasp3_24
(minor tail protein)

Nicholasp3_25

Nicholasp3_26

Nicholasp3_27
(lysin A)

Nicholasp3_28
(lysin B)

Nicholasp3_29

Nicholasp3_30

Nicholasp3_31

Nicholasp3_32
(NrdH-like glutaredoxin)

Nicholasp3_33

Nicholasp3_34

Nicholasp3_35
(nucleotidyltransferase)

Nicholasp3_36
(Lsr2-like DNA bridging protein)

Nicholasp3_37

Nicholasp3_38
(tyrosine integrase)

Nicholasp3_39

Nicholasp3_40
(immunity repressor)

Nicholasp3_41
(Cro protein)

Nicholasp3_42

Nicholasp3_43

Nicholasp3_44

Nicholasp3_45

Nicholasp3_46

Nicholasp3_47
(esterase)

Nicholasp3_48

Nicholasp3_49

Nicholasp3_50

Nicholasp3_51

Nicholasp3_52

Nicholasp3_53

Nicholasp3_54

Nicholasp3_55
(Ro-like RNA binding protein)

Nicholasp3_56

Nicholasp3_57

Nicholasp3_58

Nicholasp3_59

Nicholasp3_60

Nicholasp3_61

Nicholasp3_62

Nicholasp3_63
(ERF family ssDNA binding protein)

Nicholasp3_64

Nicholasp3_65

Nicholasp3_66

Nicholasp3_67
(HNH endonuclease)

Nicholasp3_68

Nicholasp3_69

Nicholasp3_70
(DnaB-like dsDNA helicase)

Nicholasp3_71
(WhiB family transcription factor)

Nicholasp3_72

Nicholasp3_73

Nicholasp3_74
(phosphoribosyl pyrophosphate transferase)

Nicholasp3_75
(nicotinate ribosyltransferase)

Nicholasp3_76
(polynucleotide kinase)

Nicholasp3_77

Nicholasp3_78

Nicholasp3_79

Nicholasp3_80
(RusA-like resolvase)

Nicholasp3_81
(WhiB family transcription factor)

Nicholasp3_82
(WhiB family transcription factor)

Nicholasp3_83

Nicholasp3_84

Nicholasp3_85
(ssDNA-binding protein)

Nicholasp3_86

Nicholasp3_87

Nicholasp3_88
(RNA ligase)

Nicholasp3_89

Nicholasp3_90
(ClpP-like protease)

Nicholasp3_91

Nicholasp3_92

Nicholasp3_93

Nicholasp3_94

Nicholasp3_95
(HNH endonuclease)

Nicholasp3_96

Nicholasp3_97

Nicholasp3_98
(DnaQ-like DNA polymerase III subunit)

Nicholasp3_99

Nicholasp3_100

Nicholasp3_101

Nicholasp3_102

Nicholasp3_103

Nicholasp3_104

Nicholasp3_105

Nicholasp3_106 — tRNA

Nicholasp3_107

Nicholasp3_108 — tRNA

Nicholasp3_109 — tRNA

Nicholasp3_110 — tRNA

Nicholasp3_111

Nicholasp3_112 — tRNA

Nicholasp3_113 — tRNA

Nicholasp3_114 — tRNA

Nicholasp3_115 — tRNA

Nicholasp3_116 — tRNA

Nicholasp3_117

Nicholasp3_118

Nicholasp3_119 — tRNA

Nicholasp3_120

Nicholasp3_121

Nicholasp3_122 — tRNA

Nicholasp3_123

Nicholasp3_124 — tRNA

Nicholasp3_125

Nicholasp3_126

Nicholasp3_127
(AAA-ATPase)

Nicholasp3_128

Nicholasp3_129

Nicholasp3_130

Nicholasp3_131

Nicholasp3_132

Nicholasp3_133

Nicholasp3_134

Nicholasp3_135

Nicholasp3_136

Nicholasp3_137

Nicholasp3_138

Nicholasp3_139

Nicholasp3_140

Nicholasp3_141

Nicholasp3_142

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140422
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Not in Pitt Archives
SEA Designator 2012UTEPnicholasp3ZCRO
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload