| Detailed Information for Phage Red305 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Avery Redlich |
| Year Found | 2024 |
| Location Found | Plantation, FL United States |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 33°C |
| GPS Coordinates | 26.10697 N, 80.27325 W Map |
| Discovery Notes | Not too deep in soil, still oxygenated. Low-traffic soil area. No pesticides in area. Semi-sandy. |
| Naming Notes | Phage is from South Florida, scientist is from Miami area. Area code is 305. Scientist name is Redlich, abbreviated to Red. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 20, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5576 |
| Genome length (bp) | 75628 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Brown University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Relatively clear. Small-medium size circles present. Very consistent morphology throughout testing and continued platings. |
| Morphotype | Siphoviridae |
| Number of Genes | 131 |
| Number of tRNAs | 10 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |