| Detailed Information for Phage StolenFromERC |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Erin Cafferty |
| Year Found | 2015 |
| Location Found | Cullowhee, NC USA |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.316817 N, 83.166903 W Map |
| Discovery Notes | Dirt was slightly moist; from a compost pile. |
| Naming Notes | The name StolenFromERC is from a family joke. My grandfather would label all of his things "Stolen From MPC" and my father would do the same. It is a funny name with family history. Also, my phage will never be stolen. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 4, 2020 |
| Sequencing Facility | Western Carolina University |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 202 |
| Genome length (bp) | 75270 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Allegany College of Maryland |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques moderately small, varying in size slightly. Though it appears lytic here, this phage is temperate because it forms mesas at high concentrations. |
| Morphotype | Siphoviridae |
| Number of Genes | 146 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553904 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.6×10^7 pfu/mL |
| Date of SEA Lysate Titering |
Oct 21, 2015 |
| Pitt Freezer Box# |
19 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
