| Detailed Information for Phage Tarkin |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Dion |
| Found By | Ethan Dionne |
| Year Found | 2018 |
| Location Found | Providence, RI USA |
| Finding Institution | Providence College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 41.843 N, 71.438 W Map |
| Discovery Notes | A soil sample was collected outside of the Albertus Magnus Science Building, Providence College, Providence, RI on September 5, 2018 at 4pm. The sample was slightly moist, clay-like top soil taken from a mostly shady grassy area during partly cloudy conditions, 27.8°C with 68% humidity. |
| Naming Notes | The name "Tarkin" comes from the Star Wars villain Grand Moff Tarkin, who was in charge of the Death Star's operations. I likened Tarkin's role in operating a weapon of planetary destruction to phage, which "fire" their DNA into a host, eventually resulting in the host's destruction. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 31, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 911 |
| Genome length (bp) | 75998 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Providence College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are relatively small, clear in color, and distinct against the bacterial lawn. |
| Morphotype | Siphoviridae |
| Number of Genes | 142 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ681505 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.3*10^10 |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
E2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
