| Detailed Information for Phage TeardropMSU |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Florence Ogalo and Nandini Surendranathan |
| Year Found | 2013 |
| Location Found | Montclair, NJ USA |
| Finding Institution | Montclair State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.863056 N, 74.194722 W Map |
| Discovery Notes | It was found outside of Bohn Hall, a residence hall in Montclair State University. |
| Naming Notes | This specific phage produces tear drop shaped plaques. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 61 |
| Sanger Finishing Reactions | 6 |
| Genome length (bp) | 74896 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques produced were small around 1.5 mm wide and irregularly shaped similar to teardrops. |
| Morphotype | Siphoviridae |
| Number of Genes | 143 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU865303 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2 x 10^10 pfu/ml |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
E4 |
| Available Files |
| EM Picture | Download |