Detailed Information for Phage TeardropMSU |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Florence Ogalo and Nandini Surendranathan |
Year Found | 2013 |
Location Found | Montclair, NJ USA |
Finding Institution | Montclair State University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Unknown |
Isolation Temperature | Not entered |
GPS Coordinates | 40.863056 N, 74.194722 W Map |
Discovery Notes | It was found outside of Bohn Hall, a residence hall in Montclair State University. |
Naming Notes | This specific phage produces tear drop shaped plaques. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 22, 2014 |
Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
Shotgun Sequencing Method | 454 Pyrosequencing |
Approximate Shotgun Coverage | 61 |
Sanger Finishing Reactions | 6 |
Genome length (bp) | 74896 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 9 bases |
Overhang Sequence | CGCTTGTCA |
GC Content | 63.0% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | E |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | The plaques produced were small around 1.5 mm wide and irregularly shaped similar to teardrops. |
Morphotype | Siphoviridae |
Number of Genes | 143 |
Number of tRNAs | 2 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KU865303 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
2 x 10^10 pfu/ml |
Pitt Freezer Box# |
1 |
Pitt Freezer Box Grid# |
E4 |
Available Files |
EM Picture | Download |