| Detailed Information for Phage Wyatt2 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kayla Hays and KC Bobo |
| Year Found | 2016 |
| Location Found | Corpus Christi, TX USA |
| Finding Institution | Del Mar College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 27.77 N, 97.405833 W Map |
| Discovery Notes | The soil was found outside of a flower shop. The soil was very moist on the day of discovery. |
| Naming Notes | The name comes from both of our names being Wyatt. We originally wanted it to be Wyatt squared but it was not possible on the system.
Wyatt2 was discovered by Kayla Hays and KC Bobo. However, Wyatt Hooks and Wyatt Mitchell adopted and characterized the phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2260 |
| Genome length (bp) | 74010 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGAACGGCC |
| GC Content | 58.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Smaller plaques with a high quantity. Clear edges on plaques. Lytic life cycle. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Generally the plaques are 2mm in diameter, their shape are round, and they are fairly concentrated. They have a lytic life cycle. Typically a 18-20 hour incubation time. |
| Morphotype | Siphoviridae |
| Number of Genes | 124 |
| Number of tRNAs | 8 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN586018 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
34 |
| Pitt Freezer Box Grid# |
H9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
