| Detailed Information for Phage Zakai |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Zakai Meghoo-Peddie |
| Year Found | 2015 |
| Location Found | Atlanta, GA USA |
| Finding Institution | Morehouse College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.748116 N, 84.414223 W Map |
| Discovery Notes | collected from a rocky and dry type of soil on a very warm and dry day. Using cluster specific PCR primers indicated that this is a Cluster A phage. |
| Naming Notes | The name of the phage comes from the name Zakai which means pure and innocent. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 28, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1989 |
| Genome length (bp) | 76363 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear and round plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 132 |
| Number of tRNAs | 14 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX580961 |
| Refseq Number | None yet |
| Published in Paper | Herren et al. 2017, Genome Announcements: Genome Sequences of Four Subcluster L2 Mycobacterium Phages, Finemlucis, Miley16, Wilder, and Zakai |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
21 |
| Pitt Freezer Box Grid# |
C4 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |