| Detailed Information for Phage Tuco |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Eddy Santos |
| Year Found | 2013 |
| Location Found | Nyack, NY USA |
| Finding Institution | Alliance University (formerly Nyack College) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 41.081909 N, 73.927521 W Map |
| Discovery Notes | Hee Jae Ko found this soil sample by a tree by Simpson Hall at Nyack College. This soil sample had two types of phages named Tuco and Beesknees in it. Eddy Santos worked on Tuco while Beesknees was worked on by Greg Mastrangelo. |
| Naming Notes | Tuco was a character in the TV show series Breaking Bad |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2014 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 4765 |
| Genome length (bp) | 76944 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 63.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | A lot of small and clear plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 148 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX817173 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.4x10^10 |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
C12 |
| Available Files |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |